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Literature summary extracted from

  • Wiater, A.; Szczodrak, J.
    Selection of strain and optimization of mutanase production in submerged cultures of Trichoderma harzianum (2002), Acta Biol. Hung., 53, 389-401.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.59 medicine the enzyme has a potential as a caries preventive agent, due to the ability to remove the biofilms created by oral bacteria in vitro and to reduce plaque formation in vivo Trichoderma harzianum

General Stability

EC Number General Stability Organism
3.2.1.59 the enzyme in crude state is highly stable after two treatments of freezing and thawing Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.59 extracellular
-
 Aspergillus nidulans
-
-
3.2.1.59 extracellular
-
 Trichoderma harzianum
-
-
3.2.1.59 extracellular
-
 Trichoderma reesei
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.59 Aspergillus nidulans
-
-
-
3.2.1.59 Aspergillus nidulans 1
-
-
-
3.2.1.59 Trichoderma harzianum
-
 strain F-470 is a highly effective producer of mutanase. Effective production by strain f340. Low production by strains 55A5, strain 70B5, strain 32A3 and strain 53059
-
3.2.1.59 Trichoderma reesei
-
 effective production by strain M-7, low production by strain strain F-560
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.59 cell culture submerged Aspergillus nidulans
-
3.2.1.59 cell culture submerged Trichoderma harzianum
-
3.2.1.59 cell culture submerged Trichoderma reesei
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.59 4°C, crude enzyme is stable for about 2 weeks Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.59 mutan + H2O
-
 Aspergillus nidulans ?
-
 ?
3.2.1.59 mutan + H2O
-
 Trichoderma harzianum ?
-
 ?
3.2.1.59 mutan + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.59 mutan + H2O
-
 Aspergillus nidulans 1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.59 40
-
-
 Trichoderma harzianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.59 30 50 30°C: about 75% of maximal activity, 50°C: about 30% of maximal activity Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.59 21
-
 diluted enzyme, 1:19, 0.2 M acetate bufferm pH 5.5, at room temperature is stable for 24-48 h Trichoderma harzianum
3.2.1.59 35
-
 1 h, stable up to Trichoderma harzianum
3.2.1.59 45
-
 1 h, enzyme loses 23% of its maximal activity Trichoderma harzianum
3.2.1.59 60
-
 1 h, complete loss of activity Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.59 5.5
-
-
 Trichoderma harzianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.59 4.5 7 4.5: about 35% of maximal activity, pH 7.0: about 60% of maximal activity Trichoderma harzianum