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Literature summary extracted from

  • Wadskov-Hansen, S.L.; Willemoes, M.; Martinussen, J.; Hammer, K.; Neuhard, J.; Larsen, S.
    Cloning and verification of the Lactococcus lactis pyrG gene and characterization of the gene product, CTP synthase (2001), J. Biol. Chem., 276, 38002-38009.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.2
-
 Lactococcus lactis

General Stability

EC Number General Stability Organism
6.3.4.2 the tetramer is very stable even at dilute enzyme concentrations Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.2 CTP cooperative inhibition Lactococcus lactis
6.3.4.2 dCTP very weak inhibitor Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.2 Mg2+ required, the activation curve for free Mg2+ concentration is greatly dependent on whether the concentration of ATP and UTP are near saturating Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.2 60000
-
 2 * 60000 Lactococcus lactis
6.3.4.2 234000
-
 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.2 additional information Lactococcus lactis in Lactococcus lactis the pyrG gene product is the only enzyme responsible for the amination of UTP to CTP ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.2 Lactococcus lactis
-
 subsp. cremonis
-

Storage Stability

EC Number Storage Stability Organism
6.3.4.2 -20°C, stable for several months Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.2 ATP + dUTP + NH4+ half-saturating concentration of 0.6 mM for dUTP, it is unlikely that this reaction plays a significant physiological role Lactococcus lactis ADP + phosphate + dCTP
-
 ?
6.3.4.2 ATP + UTP + NH4+
-
 Lactococcus lactis ADP + phosphate + CTP
-
 ?
6.3.4.2 additional information in Lactococcus lactis the pyrG gene product is the only enzyme responsible for the amination of UTP to CTP Lactococcus lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.2 tetramer 2 * 60000 Lactococcus lactis