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Literature summary extracted from

  • El-Said Mohamed, M.; Fuchs, G.
    Purification and characterization of phenylacetyl-coenzyme A ligase from a denitrifying Pseudomonas sp., an enzyme involved in the anaerobic degradation of phenylacetate (1993), Arch. Microbiol., 159, 544-562 (c).
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.30 enzyme is rather labile and requires the presence of glycerol and/or phenylacetate for stabilization Pseudomonas sp.
6.2.1.30 purified enzyme is sensitive to repeated freezing Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.30 Cu2+ 2 mM, 87% inhibition Pseudomonas sp.
6.2.1.30 iodoacetamide
-
 Pseudomonas sp.
6.2.1.30 Mo5+ 2 mM, 80% inhibition Pseudomonas sp.
6.2.1.30 NaF
-
 Pseudomonas sp.
6.2.1.30 NEM
-
 Pseudomonas sp.
6.2.1.30 Ni2+ 1 mM, 65% inhibition Pseudomonas sp.
6.2.1.30 p-hydroxymercuribenzoate
-
 Pseudomonas sp.
6.2.1.30 Zn2+ 2 mM, 84% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.30 0.06
-
 phenylacetate pH 7.8, 30°C Pseudomonas sp.
6.2.1.30 0.15
-
 CoA pH 7.8, 30°C Pseudomonas sp.
6.2.1.30 0.29
-
 ATP pH 7.8, 30°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.30 Mg2+ required Pseudomonas sp.
6.2.1.30 Mg2+ optimal concentration: 5 mM Pseudomonas sp.
6.2.1.30 Mn2+ at 5 mM, 41% of the activation with Mg2+ Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.30 50000
-
 1 * 50000, SDS-PAGE Pseudomonas sp.
6.2.1.30 52000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.30 ATP + phenylacetate + CoA Pseudomonas sp. first step in anaerobic degradation pathway of phenylacetate ?
-
 ?
6.2.1.30 ATP + phenylacetate + CoA Pseudomonas sp. KB 740 first step in anaerobic degradation pathway of phenylacetate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.30 Pseudomonas sp.
-
-
-
6.2.1.30 Pseudomonas sp. KB 740
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
6.2.1.30 insensitive to oxygen Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.30 strain KB 740 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.30 cell culture maximal activity in mid-exponential growth pase Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.30 24.4
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
6.2.1.30 -20°C or -70°C, 70% loss of activity after 1 week Pseudomonas sp.
6.2.1.30 4°C, 90% loss of activity after 1 day without glycerol, plus 10% glycerol, half-life: 2 days, plus 5 mM phenylacetate, half-life: 1 week, plus 35% glycerol, half-life: 2 weeks Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.30 ATP + phenylacetate + CoA highly specific Pseudomonas sp. AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA highly specific Pseudomonas sp. KB 740 AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA first step in anaerobic degradation pathway of phenylacetate Pseudomonas sp. ?
-
 ?
6.2.1.30 ATP + phenylacetate + CoA first step in anaerobic degradation pathway of phenylacetate Pseudomonas sp. KB 740 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.30 monomer 1 * 50000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.30 21.7
-
 ATP pH 7.8, 30°C Pseudomonas sp.
6.2.1.30 21.7
-
 CoA pH 7.8, 30°C Pseudomonas sp.
6.2.1.30 21.7
-
 phenylacetate pH 7.8, 30°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.30 8.5
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.30 8.5 9 pH 8.5: activity maximum, pH 9.0: 50% of maximal activity Pseudomonas sp.