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Literature summary extracted from

  • Frendo, P.; Jimenez, M.J.; Mathieu, C.; Duret, L.; Gallesi, D.; Van de Sype, G.; Herouart, D.; Puppo, A.
    A Medicago truncatula homoglutathione synthetase is derived from glutathione synthetase by gene duplication (2001), Plant Physiol., 126, 1706-1715.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 gene gshs1, construction of cDNA library, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain 830, phylogenetic analysis reveals that gshs1 and gshs2, encoding homoglutathione synthetase, are a result of gene duplication, genomic organization Medicago truncatula
6.3.2.23 expression in Escherichia coli Medicago truncatula

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.23 L534A increased production of glutathione and reduced formation of homglutathione Medicago truncatula
6.3.2.23 L534A/P535A as compared with L534A mutant, even further increased production of glutathione and further reduced formation of homglutathione Medicago truncatula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly Medicago truncatula
-
 ADP + phosphate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Medicago truncatula Q94GA0 gene gshs1, cv. Jemalong genotype J5
-
6.3.2.23 Medicago truncatula Q9XEB8
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione residues A534 and A535 are involved in substrate binding and determination of substrate specificity Medicago truncatula
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 additional information
-
-
 Medicago truncatula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly
-
 Medicago truncatula ADP + phosphate + glutathione
-
 ?
6.3.2.23 ATP + gamma-glutamylcysteine + glycine
-
 Medicago truncatula ADP + phosphate + gamma-glutamylcysteinylglycine
-
 ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Medicago truncatula ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.3 Glutathione synthetase
-
 Medicago truncatula
6.3.2.3 GS
-
 Medicago truncatula
6.3.2.3 GSHS1
-
 Medicago truncatula

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.3 ATP
-
 Medicago truncatula