Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Griffiths, E.T.; Harries, P.C.; Jeffcoat, R.; Trudgill, P.W.
    Purification and properties of a alpha-pinene oxide lyase from Nocardia sp. strain P18.3 (1987), J. Bacteriol., 169, 4980-4983.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
5.5.1.10 retains activity after prolonged dialysis Nocardia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.10 5,5'-dithiobis(2-nitrobenzoate)
-
 Nocardia sp.
5.5.1.10 beta-Pinene oxide
-
 Nocardia sp.
5.5.1.10 Carvone
-
 Nocardia sp.
5.5.1.10 limonene oxide
-
 Nocardia sp.
5.5.1.10 p-hydroxymercuribenzoate
-
 Nocardia sp.
5.5.1.10 Pinan-3-ol
-
 Nocardia sp.
5.5.1.10 Pinan-3-one
-
 Nocardia sp.
5.5.1.10 Pinane derivatives with substituent groups at carbon 3
-
 Nocardia sp.
5.5.1.10 sodium arsenite weak Nocardia sp.
5.5.1.10 Sulfhydryl reactive compounds
-
 Nocardia sp.
5.5.1.10 Terpene epoxides
-
 Nocardia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.10 0.009
-
 alpha-Pinene oxide pH 9.0, 30°C Nocardia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.5.1.10 soluble
-
 Nocardia sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.10 17000
-
 x * 17000 + x * 22000, SDS-PAGE Nocardia sp.
5.5.1.10 22000
-
 x * 17000 + x * 22000, SDS-PAGE Nocardia sp.
5.5.1.10 50000
-
 ultracentrifugal analysis Nocardia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.10 alpha-pinene oxide Nocardia sp. alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
5.5.1.10 alpha-pinene oxide Nocardia sp. P18.3 alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.10 Nocardia sp.
-
-
-
5.5.1.10 Nocardia sp. P18.3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.10 strain P18.3 Nocardia sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
5.5.1.10 alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal mechanism Nocardia sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.10 culture condition:alpha-pinene-grown cell
-
 Nocardia sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.10 312
-
-
 Nocardia sp.

Storage Stability

EC Number Storage Stability Organism
5.5.1.10 4°C, 10 days, 7% loss of activity Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.10 alpha-pinene oxide both rings of pinene are cleaved Nocardia sp. (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
5.5.1.10 alpha-pinene oxide alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene Nocardia sp. (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
5.5.1.10 alpha-pinene oxide both rings of pinene are cleaved Nocardia sp. P18.3 (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
5.5.1.10 alpha-pinene oxide alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene Nocardia sp. P18.3 (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.10 ? x * 17000 + x * 22000, SDS-PAGE Nocardia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.10 30
-
 assay at Nocardia sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.10 250
-
 alpha-Pinene oxide pH 9.0, 30°C Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.10 7 10 no significant variation in activity over this range Nocardia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
5.5.1.10 additional information enzyme is devoid of prosthetic groups and has no cofactor requirement Nocardia sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.5.1.10 Nocardia sp. isoelectric focusing
-
 4