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Literature summary extracted from

  • Henn-Sax, M.; Thoma, R.; Schmidt, S.; Hennig, M.; Kirschner, K.; Sterner, R.
    Two (betaalpha)8-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates (2002), Biochemistry, 41, 12032-12042.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.24 cloning of trpF mutants, expression in Escherichia coli BL21(DE3) Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.24 hanging drop vapor diffusion method Thermotoga maritima
5.3.1.24 X-ray structure of a complex between TrpF and its product analogue reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.16 D127N turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2481fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 3.3fold higher than that of the wild-type enzyme Thermotoga maritima
5.3.1.16 D51N turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.3fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.4fold higher than that of the wild-type enzyme Thermotoga maritima
5.3.1.16 D8N activity with N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is immeasurable low Thermotoga maritima
5.3.1.16 H48A turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 1.8fold lower than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 4.8fold lower than that of the wild-type enzyme Thermotoga maritima
5.3.1.16 R83N turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.9fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 63.2fold higher than that of the wild-type enzyme Thermotoga maritima
5.3.1.16 T164A turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 37.2fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.3fold higher than that of the wild-type enzyme Thermotoga maritima
5.3.1.24 C7A catalytically inactive mutant Thermotoga maritima
5.3.1.24 D126N mutant with drastically reduced activity Thermotoga maritima
5.3.1.24 D126N/C7A catalytically inactive double mutant Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.16 0.6
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C or 37°C, pH 7.5, wild-type enzyme Thermotoga maritima
5.3.1.16 1.6
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5 Escherichia coli
5.3.1.16 1.8
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5 Escherichia coli
5.3.1.16 2
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D127N Thermotoga maritima
5.3.1.16 2.9
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme H48A Thermotoga maritima
5.3.1.16 5.6
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme T164A Thermotoga maritima
5.3.1.16 6.4
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D51N Thermotoga maritima
5.3.1.16 37.9
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme R83N Thermotoga maritima
5.3.1.24 0.00028
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 25°C, dimer Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.16 25600
-
 analytical ultracentrifugation Thermotoga maritima
5.3.1.16 27400
-
 analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.16 additional information Escherichia coli the enzyme is involved in histidine biosynthesis ?
-
 ?
5.3.1.16 additional information Thermotoga maritima the enzyme is involved in histidine biosynthesis ?
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Thermotoga maritima involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.16 Escherichia coli
-
-
-
5.3.1.16 Thermotoga maritima
-
-
-
5.3.1.24 Thermotoga maritima Q56320 monofunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.24 TrpF mutants C7A, D126N and C7A/D126N double mutant Thermotoga maritima

Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.1.16 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed Escherichia coli
a
5.3.1.16 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed Thermotoga maritima
a
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate catalytic mechanism Thermotoga maritima
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.16 additional information enzyme catalyzes Amadori rearrangements of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is poposed Escherichia coli ?
-
 ?
5.3.1.16 additional information the enzyme is involved in histidine biosynthesis Escherichia coli ?
-
 ?
5.3.1.16 additional information the enzyme is involved in histidine biosynthesis Thermotoga maritima ?
-
 ?
5.3.1.16 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
 Escherichia coli ?
-
 ?
5.3.1.16 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
 Thermotoga maritima ?
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate high catalytic efficiency Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate reaction mechanism involving general acid-base catalysis and a Schiff base intermediate, Cys-7 and Asp-126 are important for the reaction Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.16 monomer
-
 Escherichia coli
5.3.1.16 monomer
-
 Thermotoga maritima
5.3.1.24 dimer
-
 Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
5.3.1.16 eHisA
-
 Escherichia coli
5.3.1.16 tHisA
-
 Thermotoga maritima
5.3.1.24 TrpF
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.24 25
-
 assay at Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.16 40
-
 10 min, 50% loss of the initial CD signal Escherichia coli
5.3.1.16 95
-
 10 min, 50% loss of the initial CD signal Thermotoga maritima
5.3.1.24 additional information
-
 extremely thermostable Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.16 0.00027
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D127N Thermotoga maritima
5.3.1.16 0.018
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme T164A Thermotoga maritima
5.3.1.16 0.23
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme R83N Thermotoga maritima
5.3.1.16 0.29
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D51N Thermotoga maritima
5.3.1.16 0.38
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme H48A Thermotoga maritima
5.3.1.16 0.67
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, wild-type enzyme Thermotoga maritima
5.3.1.16 1.54
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5, wild-type enzyme Thermotoga maritima
5.3.1.16 2.94
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5, wild-type enzyme Thermotoga maritima
5.3.1.16 4.9
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5 Escherichia coli
5.3.1.16 6.08
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, wild-type enzyme Thermotoga maritima
5.3.1.16 14.3
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5 Escherichia coli
5.3.1.16 32
-
 N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 80°C, pH 7.5, wild-type enzyme Thermotoga maritima
5.3.1.24 3.7
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 25°C, dimer Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.24 7.5
-
 assay at Thermotoga maritima