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Literature summary extracted from
- Phosphoribosylanthranilate isomerase and indoleglycerol-phosphate synthase: tryptophan biosynthetic enzymes from Thermotoga maritima (2001), Methods Enzymol., 331, 270-280.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.24 | trpF gene is cloned and expressed in Escherichia coli | Thermotoga maritima |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.24 | x-ray structure | Thermotoga maritima |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.3.1.24 | extremely stable enzyme | Thermotoga maritima |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.48 | phosphate | - |
Thermotoga maritima |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.48 | 0.000006 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C | Thermotoga maritima | |
| 4.1.1.48 | 0.000014 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 45°C | Thermotoga maritima | |
| 4.1.1.48 | 0.000053 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 60°C | Thermotoga maritima | |
| 4.1.1.48 | 0.000123 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 80°C | Thermotoga maritima | |
| 5.3.1.24 | 0.00028 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 25°C, dimer | Thermotoga maritima | |
| 5.3.1.24 | 0.00039 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 45°C, dimer | Thermotoga maritima | |
| 5.3.1.24 | 0.00073 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 60°C, dimer | Thermotoga maritima | |
| 5.3.1.24 | 0.00103 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 80°C, dimer | Thermotoga maritima | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.48 | 23600 | - |
gel filtration | Thermotoga maritima |
| 4.1.1.48 | 28700 | - |
1 * 28700, deduced from gene sequence | Thermotoga maritima |
| 4.1.1.48 | 31000 | - |
equilibrium sedimentation analysis | Thermotoga maritima |
| 5.3.1.24 | 23040 | - |
2 * 23040, calculated from the amino acid sequence | Thermotoga maritima |
| 5.3.1.24 | 30200 | - |
dimer, gel filtration | Thermotoga maritima |
| 5.3.1.24 | 49600 | - |
dimer, sedimentation equilibrium analysis | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Thermotoga maritima | fourth step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.48 | Thermotoga maritima | - |
recombinant enzyme, expression in Escherichia coli | - |
| 5.3.1.24 | Thermotoga maritima | - |
monofunctional enzyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.24 | recombinant PRAI, expressed in Escherichia coli | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.48 | 2.3 | - |
- |
Thermotoga maritima |
| 5.3.1.24 | 14.3 | - |
37°C | Thermotoga maritima |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.1.48 | 4°C, 1 mM potassium phosphate buffer pH 7.5, 1 mM dithiothreitol, stable for hours | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.48 | 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Thermotoga maritima | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
ir | |
| 5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Thermotoga maritima | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
ir | |
| 5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | fourth step in tryptophan biosynthesis | Thermotoga maritima | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.48 | monomer | 1 * 28700, deduced from gene sequence | Thermotoga maritima |
| 5.3.1.24 | homodimer | 2 * 23040, calculated from the amino acid sequence | Thermotoga maritima |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.48 | 84 | - |
110 min, half-life for irreversible thermal denaturation | Thermotoga maritima |
| 5.3.1.24 | additional information | - |
structural features resonsible for the high thermostability | Thermotoga maritima |
| 5.3.1.24 | 85 | - |
dimer, half-life: 310 min | Thermotoga maritima |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.24 | 3.7 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 25°C, dimer | Thermotoga maritima | |
| 5.3.1.24 | 13.5 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 45°C, dimer | Thermotoga maritima | |
| 5.3.1.24 | 38.5 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 60°C, dimer | Thermotoga maritima | |
| 5.3.1.24 | 130 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 80°C, dimer | Thermotoga maritima | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.48 | 7 | 7.5 | - |
Thermotoga maritima |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.48 | 0.27 | - |
phosphate | 60°C | Thermotoga maritima | |
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