Literature summary extracted from
Kammen, H.; Marvel, C.; Hardy, L.; Penhoet, E.
Purification structure and properties of Escherichia coli tRNA pseudouridine synthase I (1988), J. Biol. Chem., 263, 2255-2263.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.4.99.12 |
Dithionitrobenzoate |
irreversibly inactivates |
Escherichia coli |
|
5.4.99.12 |
iodoacetate |
irreversibly inactivates |
Escherichia coli |
|
5.4.99.12 |
p-chloromercuribenzoate |
irreversibly inactivates |
Escherichia coli |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.4.99.12 |
31000 |
- |
x * 31000, SDS-PAGE |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.99.12 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.99.12 |
isolation of virtually homogeneous tRNA pseudouridine synthase I from strains of Escherichia coli transformed with plasmid in which the production of tRNA pseudouridine synthase I is amplified 20fold |
Escherichia coli |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
5.4.99.12 |
-20°C or 2°C, 50% w/v glycerol, 50% loss of activity after 2 years |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.4.99.12 |
tRNA uridine38-40 |
purified tRNA pseudouridine synthase I modifies all of the hisT isoacceptors of tRNAHis, tRNATyr, and tRNALeu to products which are chromatographically indistinguishable from the respective wild-type species. These three groups of isoacceptors contain all the known topological sites for pseudouridine modification of residues 38,39, and 40 |
Escherichia coli |
tRNA pseudouridine38-40 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.4.99.12 |
? |
x * 31000, SDS-PAGE |
Escherichia coli |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.4.99.12 |
hisT |
- |
Escherichia coli |
5.4.99.12 |
pseudouridine synthase I |
- |
Escherichia coli |