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Literature summary extracted from

  • Schenzle, A.; Lenke, H.; Spain, J.C.; Knackmuss, H.J.
    3-Hydroxylaminophenol mutase from Ralstonia eutropha JMP134 catalyzes a Bamberger rearrangement (1999), J. Bacteriol., 181, 1444-1450.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.4.3 1,10-phenanthroline weak Cupriavidus necator
5.4.4.3 3-nitrosophenol autoxidation product of substrate 3-hydroxyaminophenol, inhibition can be partly reversed by addition of 2 mM DTT and/or 10 mM hydroxylamine Cupriavidus necator
5.4.4.3 AgNO3 at 1 mM Cupriavidus necator
5.4.4.3 H2O2 at 0.0003 mM Cupriavidus necator
5.4.4.3 L-cysteine 39% loss of activity after 90 min, 83% loss of activity after 240 min Cupriavidus necator
5.4.4.3 additional information not: EDTA, tiron, 10 mM DTT, 0.026 mM NaBH4, 0.1 mM KOCN, 0.1 mM NaN3, Co2+, Cu2+, Fe2+, Fe3+, Mg2+, Mn2+, Ni2+, Zn2+, NaNO2, hydroxylamine Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.4.3 0.1
-
 3-hydroxyaminophenol pH 7.0, on ice Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.4.3 additional information metal cations play no role in the reaction mechanism Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.4.3 62000
-
 x * 62000, SDS-PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.4.3 3-hydroxyaminophenol Cupriavidus necator the enzyme is involved in the degradative pathway of 3-nitrophenol aminohydroquinone
-
 ?
5.4.4.3 3-hydroxyaminophenol Cupriavidus necator JMP 134-1 the enzyme is involved in the degradative pathway of 3-nitrophenol aminohydroquinone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.4.3 Cupriavidus necator
-
 JMP134
-
5.4.4.3 Cupriavidus necator JMP 134-1
-
 JMP134
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.4.3
-
 Cupriavidus necator

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.4.3 3-hydroxyaminophenol = aminohydroquinone enzyme requires neither a cofactor nor oxygen for the enzymatic reaction, enzyme catalyzes a Bamberg-type rearrangement Cupriavidus necator
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.4.3 culture condition:3-nitrophenol grown-cell
-
 Cupriavidus necator
-
5.4.4.3 culture condition:succinate-grown cell
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.4.3 4.7
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
5.4.4.3 -70°C, 7% loss of activity, after 30 days Cupriavidus necator
5.4.4.3 storage on ice, 13% loss of activity after 14 days Cupriavidus necator
5.4.4.3 storage on ice, 37% loss of activity after 38 days Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.4.3 2-chloro-5-hydroxylaminophenol
-
 Cupriavidus necator 2-amino-5-chlorohydroquinone
-
 ?
5.4.4.3 2-chloro-5-hydroxylaminophenol
-
 Cupriavidus necator JMP 134-1 2-amino-5-chlorohydroquinone
-
 ?
5.4.4.3 3-hydroxyaminophenol the enzymatic reaction is regiospecific: the enzyme directs the formation of aminohydroquinone exclusively, whereas the chemical reaction forms the isomeric 4-aminocatechol Cupriavidus necator aminohydroquinone
-
 ?
5.4.4.3 3-hydroxyaminophenol the enzyme is involved in the degradative pathway of 3-nitrophenol Cupriavidus necator aminohydroquinone
-
 ?
5.4.4.3 3-hydroxyaminophenol the enzymatic reaction is regiospecific: the enzyme directs the formation of aminohydroquinone exclusively, whereas the chemical reaction forms the isomeric 4-aminocatechol Cupriavidus necator JMP 134-1 aminohydroquinone
-
 ?
5.4.4.3 3-hydroxyaminophenol the enzyme is involved in the degradative pathway of 3-nitrophenol Cupriavidus necator JMP 134-1 aminohydroquinone
-
 ?
5.4.4.3 4-hydroxyaminotoluene
-
 Cupriavidus necator 6-amino-m-cresol
-
 ?
5.4.4.3 hydroxylaminobenzene
-
 Cupriavidus necator 2-aminophenol + 4-aminophenol
-
 ?
5.4.4.3 hydroxylaminobenzene
-
 Cupriavidus necator JMP 134-1 2-aminophenol + 4-aminophenol
-
 ?
5.4.4.3 additional information not: 4-hydroxylaminobenzoate Cupriavidus necator ?
-
 ?
5.4.4.3 additional information not: 4-hydroxylaminobenzoate Cupriavidus necator JMP 134-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.4.3 ? x * 62000, SDS-PAGE Cupriavidus necator

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.4.3 60
-
 1 min: 72% loss of activity, 8 min: complete loss of activity Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.4.3 6.5
-
-
 Cupriavidus necator

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.4.4.3 Cupriavidus necator chromatofocusing
-
 4.5