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Literature summary extracted from
- Characterization of Styrene Oxide Isomerase, a Key Enzyme of Styrene and Styrene Oxide Metabolism in Corynehacterium sp. (1997), Biosci. Biotechnol. Biochem., 61, 2058-2062.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.3.99.7 | synthesis | the highly stereospecific enzyme can be used in a biotransformation system producing (R)-styrene oxide | Corynebacterium sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.7 | AgNO3 | 0.1 mM, 29% inhibition | Corynebacterium sp. |  |
| 5.3.99.7 | dithiothreitol | 1 mM, 22% inhibition | Corynebacterium sp. | |
| 5.3.99.7 | HgCl2 | 1 mM, 80% inhibition | Corynebacterium sp. | |
| 5.3.99.7 | hydrazine | 1 mM, 82% inhibition | Corynebacterium sp. | |
| 5.3.99.7 | hydroxylammonium chloride | 1 mM, 77% inhibition | Corynebacterium sp. | |
| 5.3.99.7 | iodoacetate | 1 mM, 80% inhibition | Corynebacterium sp. | |
| 5.3.99.7 | KCN | 1 mM, 32% inhibition | Corynebacterium sp. | |
| 5.3.99.7 | PCMB | 1 mM, 24% inhibition | Corynebacterium sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.99.7 | 0.077 | - |
styrene oxide | pH 7.0, 25°C | Corynebacterium sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.99.7 | additional information | not membrane-bound, probably bound to the cell wall | Corynebacterium sp. | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.7 | additional information | non-metal enzyme | Corynebacterium sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.7 | styrene oxide + H2O | Corynebacterium sp. | key enzyme of styrene and styrene oxide metabolism | phenylacetaldehyde | - |
ir | |
| 5.3.99.7 | styrene oxide + H2O | Corynebacterium sp. AC-5 | key enzyme of styrene and styrene oxide metabolism | phenylacetaldehyde | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.99.7 | Corynebacterium sp. | - |
- |
- |
| 5.3.99.7 | Corynebacterium sp. AC-5 | - |
- |
- |
| 5.3.99.7 | Pseudomonas sp. | - |
- |
- |
| 5.3.99.7 | Pseudomonas sp. SK3 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.99.7 | - |
Corynebacterium sp. |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.99.7 | cell culture | cells grown in a styrene gas atmosphere | Corynebacterium sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.7 | 58.7 | - |
- |
Corynebacterium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.7 | styrene oxide + H2O | compared with the (R)-isomer, the relative activity of the enzyme towards (R)-styrene oxide is 51% of that for the (S)-isomer | Corynebacterium sp. | phenylacetaldehyde | - |
? | |
| 5.3.99.7 | styrene oxide + H2O | key enzyme of styrene and styrene oxide metabolism | Corynebacterium sp. | phenylacetaldehyde | - |
ir | |
| 5.3.99.7 | styrene oxide + H2O | compared with the (R)-isomer, the relative activity of the enzyme towards (R)-styrene oxide is 51% of that for the (S)-isomer | Corynebacterium sp. AC-5 | phenylacetaldehyde | - |
? | |
| 5.3.99.7 | styrene oxide + H2O | key enzyme of styrene and styrene oxide metabolism | Corynebacterium sp. AC-5 | phenylacetaldehyde | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.7 | 40 | - |
- |
Corynebacterium sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.7 | 40 | - |
pH 7.0, 1 h, stable | Corynebacterium sp. |
| 5.3.99.7 | 50 | - |
pH 7.0, 1 h, 10% loss of activity | Corynebacterium sp. |
| 5.3.99.7 | 55 | - |
pH 7.0, 1 h, 50% loss of activity | Corynebacterium sp. |
| 5.3.99.7 | 60 | - |
pH 7.0, 1 h, 80% loss of activity | Corynebacterium sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.7 | 5 | 7.5 | - |
Corynebacterium sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.7 | 5 | 10 | pH 5.0-7.5: maximal activity, pH 10.0: about 50% of maximal activity | Corynebacterium sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.7 | 6 | - |
25°C, 1 h, unstable below | Corynebacterium sp. |
| 5.3.99.7 | 7 | 8 | 25°C, 1 h | Corynebacterium sp. |
| 5.3.99.7 | 10 | - |
25°C, 1 h, 10% loss of activity | Corynebacterium sp. |
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