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Literature summary extracted from

  • Fouchier, F.; Baltz, T.; Rougon, G.
    Identification of glycosylphosphatidylinositol-specific phospholipases C in mouse brain membranes (1990), Biochem. J., 269, 321-327.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.14 butanol activation, 2% v/v, acidic enzyme form, not neutral enzyme form Mus musculus
4.6.1.14 deoxycholate activation, 0.5-1 mg/ml, neutral enzyme form, not acidic enzyme form, inhibits above 1 mg/ml Mus musculus
4.6.1.14 additional information 2 enzyme forms differing in pH-optima and response to stimulation by butanol and deoxycholate Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.14 cetrimide i.e. alkyltrimethylammonium bromide, 0.3 mg/ml, neutral enzyme form Mus musculus
4.6.1.14 deoxycholate neutral enzyme form, above 1 mg/ml, activation at 0.5-1 mg/ml Mus musculus
4.6.1.14 EGTA Ca2+ reverses, only acidic, not neutral enzyme form Mus musculus
4.6.1.14 additional information no inhibition by Mn2+ Mus musculus
4.6.1.14 Nonidet P-40 above 0.1% w/v, neutral enzyme form Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.14 cytoplasmic membrane neutral enzyme form Mus musculus
-
-
4.6.1.14 lysosome predominantly acidic enzyme form Mus musculus 5764
-
4.6.1.14 membrane
-
 Mus musculus 16020
-
4.6.1.14 additional information subcellular localization in mouse brain membranes Mus musculus
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.14 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.14 brain
-
 Mus musculus
-
4.6.1.14 myelin neutral enzyme form Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.14 variant-surface-glycoprotein highly specific Mus musculus 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein
-
 ?
4.6.1.14 variant-surface-glycoprotein isolated from Trypanosoma equiperdum BoTat-1 Mus musculus 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.14 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.14 4.5 5 acidic enzyme form Mus musculus
4.6.1.14 6.5 7 neutral enzyme form Mus musculus