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Literature summary extracted from

  • Chocat, P.; Esaki, N.; Tanizawa, K.; Nakamura, T.; Tanaka, H.; Soda, K.
    Purification and characterization of selenocysteine beta-lyase from Citrobacter freundii (1985), J. Bacteriol., 163, 669-676.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.16 alpha-Keto acids restore activity during reaction Citrobacter freundii

General Stability

EC Number General Stability Organism
4.4.1.16 labile during purification, 0.1% 2-mercaptoethanol, 1 mM dithiothreitol or 1 M sucrose stabilizes activity Citrobacter freundii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.16 beta-chloro-L-alanine irreversible Citrobacter freundii
4.4.1.16 Hg2+
-
 Citrobacter freundii
4.4.1.16 iodoacetamide 1 mM, complete inactivation within 3 h Citrobacter freundii
4.4.1.16 iodoacetic acid 1 mM, complete inactivation within 3 h Citrobacter freundii
4.4.1.16 L-cysteine competitive Citrobacter freundii
4.4.1.16 L-cysteine methyl ester competitive Citrobacter freundii
4.4.1.16 N-ethylmaleimide 1 mM, complete inactivation within 3 h Citrobacter freundii
4.4.1.16 NaBH4 irreversible Citrobacter freundii
4.4.1.16 Ni2+
-
 Citrobacter freundii
4.4.1.16 Pb2+
-
 Citrobacter freundii
4.4.1.16 Zn2+
-
 Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.16 0.95
-
 L-selenocysteine pH 7.2 Citrobacter freundii
4.4.1.16 3.1
-
 beta-chloro-L-alanine pH 7.2 Citrobacter freundii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.16 cytoplasm
-
 Citrobacter freundii 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.16 63000
-
 HPLC gel filtration Citrobacter freundii
4.4.1.16 64000
-
 SDS-PAGE Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor Citrobacter freundii
-
 selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.16 Citrobacter freundii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.16
-
 Citrobacter freundii

Storage Stability

EC Number Storage Stability Organism
4.4.1.16 -20°C, 1 month, 60% loss of activity Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.16 beta-chloro-L-alanine alpha,beta elimination, irreversible inactivation of enzyme during the course of reaction Citrobacter freundii NH3 + pyruvate + chloride
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Citrobacter freundii selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor strictly specific, no activity with D-isomer, enzyme is inativated during course of reaction, inactivation can be prevented by addition of alpha-keto acids Citrobacter freundii selenide + L-alanine + acceptor formation of H2Se by nonenzymatic reduction of elemental selenium in presence of dithiothreitol ?
4.4.1.16 L-selenocysteine + reduced acceptor enzyme bound pyridoxal 5'-phosphate serves as acceptor Citrobacter freundii selenide + L-alanine + acceptor formation of H2Se by nonenzymatic reduction of elemental selenium in presence of dithiothreitol ?

Subunits

EC Number Subunits Comment Organism
4.4.1.16 monomer 1 * 64000, SDS-PAGE Citrobacter freundii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.16 7
-
-
 Citrobacter freundii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.16 5.5 9
-
 Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.16 pyridoxal 5'-phosphate required Citrobacter freundii
4.4.1.16 pyridoxal 5'-phosphate 1 mol bound per mol enzyme (subunit) Citrobacter freundii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.16 0.6
-
 L-cysteine methyl ester pH 7.2 Citrobacter freundii
4.4.1.16 0.65
-
 L-cysteine pH 7.2 Citrobacter freundii
4.4.1.16 2.5
-
 beta-chloro-L-alanine pH 7.2 Citrobacter freundii