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Literature summary extracted from
- Purification and properties of strictosidine synthetase (an enzyme condensing tryptamine and secologanin) from Catharanthus roseus cultured cell (1979), Biochemistry, 18, 3760-3763.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.3.2 | 0.46 | - |
secologanin | pH 7.5, 30°C | Catharanthus roseus |  |
| 4.3.3.2 | 0.83 | - |
tryptamine | pH 7.5, 30°C | Catharanthus roseus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 38000 | - |
gel filtration | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.3.2 | tryptamine + secologanin | Catharanthus roseus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.3.2 | Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.3.2 | - |
Catharanthus roseus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.3.3.2 | cell culture | - |
Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 0.35 | - |
purified enzyme, pH 7.5, 30°C | Catharanthus roseus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.3.3.2 | -20°C, 1 week, 50% loss of activity | Catharanthus roseus |
| 4.3.3.2 | 4°C, partially purified enzyme, stable for more than one week | Catharanthus roseus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.3.2 | tryptamine + secologanin | - |
Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
| 4.3.3.2 | tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 5 | 7.5 | - |
Catharanthus roseus |
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