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Literature summary extracted from

  • Treimer, J.F.; Zenk, M.H.
    Purification and properties of strictosidine synthase, the key enzyme in indole alkaloid formation (1979), Eur. J. Biochem., 101, 225-233.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.3.3.2 high loss of activity on repeated freezing and thawing Catharanthus roseus
4.3.3.2 stable on repeated freezing and thawing Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.2 additional information no inhibition with secologanin Catharanthus roseus
4.3.3.2 p-chloromercuribenzoate
-
 Amsonia orientalis
4.3.3.2 p-chloromercuribenzoate
-
 Amsonia salicifolia
4.3.3.2 p-chloromercuribenzoate
-
 Catharanthus roseus
4.3.3.2 p-chloromercuribenzoate
-
 Ochrosia elliptica
4.3.3.2 p-chloromercuribenzoate
-
 Rauvolfia vomitoria
4.3.3.2 p-chloromercuribenzoate
-
 Stemmadenia tomentosa var. palmeri
4.3.3.2 p-chloromercuribenzoate
-
 Vinca major
4.3.3.2 p-chloromercuribenzoate
-
 Voacanga africana
4.3.3.2 tryptamine inhibitory above 1.2 mM Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.2 2.3
-
 tryptamine pH 7.0, 35°C Catharanthus roseus
4.3.3.2 3.4
-
 secologanin pH 7.0, 35°C Catharanthus roseus
4.3.3.2 3.5
-
 dihydrosecologanin pH 7.0, 35°C Catharanthus roseus
4.3.3.2 8
-
 3'-O-methylsecologanin pH 7.0, 35°C Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.3.3.2 soluble
-
 Catharanthus roseus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.2 34000
-
 gel filtration Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.2 tryptamine + secologanin Catharanthus roseus involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Amsonia salicifolia involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Catharanthus pusillus involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Catharanthus trichophyllus involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Rauvolfia vomitoria involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Amsonia orientalis involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Vinca major involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Rauvolfia verticillata involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Vinca minor involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Amsonia tabernaemontana involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Catharanthus longifolius involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Ervatamia divaricatum involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Ochrosia elliptica involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Stemmadenia tomentosa var. palmeri involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin Voacanga africana involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Amsonia orientalis
-
-
-
4.3.3.2 Amsonia salicifolia
-
-
-
4.3.3.2 Amsonia tabernaemontana
-
-
-
4.3.3.2 Catharanthus longifolius
-
-
-
4.3.3.2 Catharanthus pusillus
-
-
-
4.3.3.2 Catharanthus roseus
-
 i.e. Vinca rosea
-
4.3.3.2 Catharanthus trichophyllus
-
-
-
4.3.3.2 Ervatamia divaricatum
-
-
-
4.3.3.2 Ochrosia elliptica
-
-
-
4.3.3.2 Rauvolfia verticillata
-
-
-
4.3.3.2 Rauvolfia vomitoria
-
-
-
4.3.3.2 Stemmadenia tomentosa var. palmeri
-
-
-
4.3.3.2 Vinca major
-
-
-
4.3.3.2 Vinca minor
-
-
-
4.3.3.2 Voacanga africana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2
-
 Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.2 cell culture
-
 Catharanthus roseus
-
4.3.3.2 cell culture
-
 Amsonia salicifolia
-
4.3.3.2 cell culture
-
 Rauvolfia vomitoria
-
4.3.3.2 cell culture
-
 Amsonia orientalis
-
4.3.3.2 cell culture
-
 Vinca major
-
4.3.3.2 cell culture
-
 Ochrosia elliptica
-
4.3.3.2 cell culture
-
 Stemmadenia tomentosa var. palmeri
-
4.3.3.2 cell culture
-
 Voacanga africana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.2 1.07
-
 purified enzyme, pH 7.0, 35°C Catharanthus roseus

Storage Stability

EC Number Storage Stability Organism
4.3.3.2 -20°, 1 year, no loss of activity Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 5-fluorotryptamine + secologanin 6% of the activity with trypamine Catharanthus roseus ?
-
 ?
4.3.3.2 5-hydroxytryptamine + secologanin 4% of the activity with trypamine Catharanthus roseus ?
-
 ?
4.3.3.2 6-hydroxytryptamine + secologanin 9% of the activity with trypamine Catharanthus roseus ?
-
 ?
4.3.3.2 7-fluorotryptamine + secologanin 8% of the activity with trypamine Catharanthus roseus ?
-
 ?
4.3.3.2 7-methyltryptamine + secologanin 15% of the activity with trypamine Catharanthus roseus ?
-
 ?
4.3.3.2 tryptamine + 2'-O-methylsecologanin
-
 Catharanthus roseus ?
-
 ?
4.3.3.2 tryptamine + 3'-O-methylsecologanin
-
 Catharanthus roseus ?
-
 ?
4.3.3.2 tryptamine + dihydrosecologanin
-
 Catharanthus roseus ?
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Amsonia salicifolia 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Catharanthus pusillus 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Catharanthus trichophyllus 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Rauvolfia vomitoria 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Amsonia orientalis 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Vinca major 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Rauvolfia verticillata 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Vinca minor 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Amsonia tabernaemontana 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Catharanthus longifolius 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Ervatamia divaricatum 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Ochrosia elliptica 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Stemmadenia tomentosa var. palmeri 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin
-
 Voacanga africana 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin highly specific Catharanthus roseus 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Catharanthus roseus 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Amsonia salicifolia 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Catharanthus pusillus 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Catharanthus trichophyllus 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Rauvolfia vomitoria 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Amsonia orientalis 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Vinca major 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Rauvolfia verticillata 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Vinca minor 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Amsonia tabernaemontana 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Catharanthus longifolius 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Ervatamia divaricatum 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Ochrosia elliptica 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Stemmadenia tomentosa var. palmeri 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Voacanga africana 3-alpha(S)-strictosidine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.2 45
-
-
 Catharanthus roseus
4.3.3.2 45
-
-
 Amsonia salicifolia
4.3.3.2 45
-
-
 Catharanthus pusillus
4.3.3.2 45
-
-
 Catharanthus trichophyllus
4.3.3.2 45
-
-
 Vinca major
4.3.3.2 45
-
-
 Rauvolfia verticillata
4.3.3.2 45
-
-
 Vinca minor
4.3.3.2 45
-
-
 Amsonia tabernaemontana
4.3.3.2 45
-
-
 Catharanthus longifolius
4.3.3.2 45
-
-
 Ervatamia divaricatum
4.3.3.2 45
-
-
 Ochrosia elliptica
4.3.3.2 45
-
-
 Stemmadenia tomentosa var. palmeri
4.3.3.2 50
-
-
 Rauvolfia vomitoria
4.3.3.2 50
-
-
 Amsonia orientalis
4.3.3.2 50
-
-
 Voacanga africana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.2 6
-
-
 Catharanthus roseus
4.3.3.2 6
-
-
 Amsonia orientalis
4.3.3.2 6.8
-
-
 Catharanthus roseus
4.3.3.2 6.9
-
-
 Catharanthus pusillus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.3.2 4 8
-
 Catharanthus roseus