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Literature summary extracted from
- Drosophila apurinic/apyrimidinic DNA endonucleases. Characterization of mechanism of action and demonstration of a novel type of enzyme activation (1986), J. Biol. Chem., 261, 3222-3228.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.18 | 63000 | - |
1 * 63000, AP endonuclease II, SDS-PAGE | Drosophila melanogaster |
| 4.2.99.18 | 66000 | - |
1 * 66000, AP endonuclease I, SDS-PAGE | Drosophila melanogaster |
| 4.2.99.18 | 67000 | - |
AP endonuclease II, glycerol density gradient sedimentation | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.99.18 | DNA | Drosophila melanogaster | - |
fragments of DNA | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.99.18 | Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.99.18 | AP endonuclease I and II, 400fold | Drosophila melanogaster |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.18 | additional information | - |
- |
Drosophila melanogaster |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.99.18 | DNA | - |
Drosophila melanogaster | fragments of DNA | - |
? | |
| 4.2.99.18 | DNA | AP endonuclease I forms deoxyribose 3'-phosphate and 5'-OH termini upon cleaving depurinated DNA | Drosophila melanogaster | fragments of DNA | - |
? | |
| 4.2.99.18 | DNA | cleaves the phosphodiester bond 3' to the apurinic/apyrimidinic site | Drosophila melanogaster | fragments of DNA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.99.18 | monomer | 1 * 66000, AP endonuclease I, SDS-PAGE | Drosophila melanogaster |
| 4.2.99.18 | monomer | 1 * 63000, AP endonuclease II, SDS-PAGE | Drosophila melanogaster |
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