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Literature summary extracted from

  • Cane, D.E.; Pargellis, C.
    Partial purification and characterization of pentalenene synthase (1987), Arch. Biochem. Biophys., 254, 421-429.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.7 diphosphate plus pentalene: no inhibition alone, both products bind cooperatively at the active site Streptomyces sp.
4.2.3.7 Mn2+ divalent metal required, Mg2+ or Mn2+, inhibition above 2.5 mM Streptomyces sp.
4.2.3.7 Pentalenene plus diphosphate no inhibition alone, both products bind cooperatively at the active site Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.7 0.00077
-
(E,E)-farnesyl diphosphate 30°C, pH 8.4 Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.7 Mg2+ divalent metal required, Mg2+ or Mn2+ Streptomyces sp.
4.2.3.7 Mn2+ divalent metal required, Mg2+ or Mn2+, inhibition above 2.5 mM Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.7 57000
-
-
Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.7 farnesyl diphosphate Streptomyces sp.
-
pentalenene + diphosphate
-
?
4.2.3.7 farnesyl diphosphate Streptomyces sp. UC5319
-
pentalenene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.7 Streptomyces sp.
-
UC5319
-
4.2.3.7 Streptomyces sp. UC5319
-
UC5319
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.7 partial Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.7 0.287
-
30°C, pH 8.4 Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
4.2.3.7 4°C, crude enzyme stable for up to 6 days Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.7 2-trans,6-trans-farnesyl diphosphate
-
Streptomyces sp. pentalenene + diphosphate
-
?
4.2.3.7 2-trans,6-trans-farnesyl diphosphate
-
Streptomyces sp. UC5319 pentalenene + diphosphate
-
?
4.2.3.7 farnesyl diphosphate
-
Streptomyces sp. pentalenene + diphosphate
-
?
4.2.3.7 farnesyl diphosphate trans,trans Streptomyces sp. pentalenene + diphosphate
-
?
4.2.3.7 farnesyl diphosphate
-
Streptomyces sp. UC5319 pentalenene + diphosphate
-
?
4.2.3.7 farnesyl diphosphate trans,trans Streptomyces sp. UC5319 pentalenene + diphosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.7 30
-
assay at Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.7 8.4
-
-
Streptomyces sp.