EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.12 | EDTA | 1-5 mM, 110-120% activation | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.12 | expression in Escherichia coli | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.12 | Ca2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
4.2.3.12 | Co2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
4.2.3.12 | Cu2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
4.2.3.12 | EDTA | above 10 mM | Escherichia coli | |
4.2.3.12 | EDTA | - |
Homo sapiens | |
4.2.3.12 | Mg2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
4.2.3.12 | Mn2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
4.2.3.12 | Ni2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
4.2.3.12 | Zn2+ | 10 mM, 40-100% inhibition | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.12 | 0.92 | - |
sepiapterin | pH 7.5, 37ºC | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.12 | additional information | no metal ion required | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.12 | 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | Escherichia coli | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
4.2.3.12 | 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | Homo sapiens | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.12 | Drosophila melanogaster | - |
- |
- |
4.2.3.12 | Escherichia coli | - |
- |
- |
4.2.3.12 | Homo sapiens | - |
- |
- |
4.2.3.12 | Synechocystis sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.12 | extraction, chromatography on a column of Ni-NTA gel | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 0.00023 | - |
SSCR activity | Homo sapiens |
4.2.3.12 | 0.00034 | - |
SSCR activity | Drosophila melanogaster |
4.2.3.12 | 0.00039 | - |
PTPS activity | Synechocystis sp. |
4.2.3.12 | 0.00113 | - |
PTPS activity | Escherichia coli |
4.2.3.12 | 0.00891 | - |
PTPS activity | Drosophila melanogaster |
4.2.3.12 | 0.01303 | - |
PTPS activity | Homo sapiens |
4.2.3.12 | 0.09767 | - |
SSCR activity | Synechocystis sp. |
4.2.3.12 | 0.09936 | - |
SSCR activity | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.3.12 | -70ºC, stable | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.12 | 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | Escherichia coli | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
4.2.3.12 | 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | Homo sapiens | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
4.2.3.12 | sepiapterin | the enzyme cleaves the C6 side chain of sepiapterin, sepiapterin side chain releasing activity, SSCR activity | Escherichia coli | 7,8-dihydropterin | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 60 | 80 | 3.2fold increase in activity compared to that at 37°C | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 80 | - |
- |
Homo sapiens |
4.2.3.12 | 80 | - |
24% retention of activity after 10 min | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 6.5 | 7 | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.12 | additional information | no cofactor required | Escherichia coli |