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Literature summary extracted from

  • Park, Y.S.; Kim, J.H.; Jacobson, K.B.; Yim, J.J.
    Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from Drosophila melanogaster (1990), Biochim. Biophys. Acta, 1038, 186-194.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.12 0.1
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Drosophila melanogaster
4.2.3.12 0.1
-
 7,8-dihydroneopterin triphosphate pH 7.5, 37ºC Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.12 37500
-
 x * 37500, SDS-PAGE Drosophila melanogaster
4.2.3.12 37500
-
 2 * 37500, also evidence for higher multimeric forms, SDS-PAGE Drosophila melanogaster
4.2.3.12 83000
-
 gel filtration of active enzyme seems to have larger molecular mass forms Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.12 7,8-dihydroneopterin triphosphate Drosophila melanogaster assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 additional information Drosophila melanogaster the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.12 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.12 ammonium sulfate precipitation, Affigel Blue chromatography, DEAE-Bio-gel chromatography, Affigel Blue chromatography, SDS-PAGE Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.12 head
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.12 0.0632
-
-
 Drosophila melanogaster

Storage Stability

EC Number Storage Stability Organism
4.2.3.12 -20°C, PIPES 50 mM, stable for 1 year Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.12 7,8-dihydroneopterin triphosphate
-
 Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate assay at Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 additional information the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain Drosophila melanogaster ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.12 ? x * 37500, SDS-PAGE Drosophila melanogaster
4.2.3.12 dimer 2 * 37500, also evidence for higher multimeric forms, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.12 37
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.12 7.5
-
 assay at Drosophila melanogaster