EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.12 | 0.1 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Drosophila melanogaster | |
4.2.3.12 | 0.1 | - |
7,8-dihydroneopterin triphosphate | pH 7.5, 37ºC | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 37500 | - |
x * 37500, SDS-PAGE | Drosophila melanogaster |
4.2.3.12 | 37500 | - |
2 * 37500, also evidence for higher multimeric forms, SDS-PAGE | Drosophila melanogaster |
4.2.3.12 | 83000 | - |
gel filtration of active enzyme seems to have larger molecular mass forms | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.12 | 7,8-dihydroneopterin triphosphate | Drosophila melanogaster | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
4.2.3.12 | additional information | Drosophila melanogaster | the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.12 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.12 | ammonium sulfate precipitation, Affigel Blue chromatography, DEAE-Bio-gel chromatography, Affigel Blue chromatography, SDS-PAGE | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.3.12 | head | - |
Drosophila melanogaster | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 0.0632 | - |
- |
Drosophila melanogaster |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.3.12 | -20°C, PIPES 50 mM, stable for 1 year | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.12 | 7,8-dihydroneopterin triphosphate | - |
Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
4.2.3.12 | 7,8-dihydroneopterin triphosphate | assay at | Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
4.2.3.12 | additional information | the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain | Drosophila melanogaster | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.3.12 | ? | x * 37500, SDS-PAGE | Drosophila melanogaster |
4.2.3.12 | dimer | 2 * 37500, also evidence for higher multimeric forms, SDS-PAGE | Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 37 | - |
assay at | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.12 | 7.5 | - |
assay at | Drosophila melanogaster |