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Literature summary extracted from

  • Inoue, Y.; Kawasaki, Y.; Harada, T.; Hatekeyama, K.; Kagamiyama, H.
    Purification and cDNA cloning of rat 6-pyruvoyl-tetrahydropterin synthase (1991), J. Biol. Chem., 266, 20791-20796.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.12 dithioerythritol increased catalytic activity with 10 mM Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.12 cloned Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.12 ammonium sulfate 50% inactivation at 400 mM Rattus norvegicus
4.2.3.12 additional information NaCl 100 mM and KCl 100 mM have no effect on activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.12 0.0091
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Rattus norvegicus
4.2.3.12 0.0091
-
 7,8-dihydroneopterin triphosphate pH 7.4, 37ºC Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.12 Ca2+ 65% of the activity with equal concentration of Mg2+ Rattus norvegicus
4.2.3.12 Mg2+ optimum concentration is 8 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.12 17000
-
 x * 17000, SDS-PAGE Rattus norvegicus
4.2.3.12 17000
-
 4 * 17000, SDS-PAGE Rattus norvegicus
4.2.3.12 83000
-
 HPLC gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.12 7,8-dihydroneopterin triphosphate Rattus norvegicus assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.12 Rattus norvegicus P27213 Wistar, cloned
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.12 additional information mature protein lacks 4 N-terminal amino acids residues Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.12 ammonium sulfate precipitation, hydroxyapatite column, butyl-toyopearl chromatography, gel filtration, HPLC ion exchange column Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.12 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.12 0.2883
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
4.2.3.12 -70°C, stable for at least 1 year Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.12 7,8-dihydroneopterin triphosphate
-
 Rattus norvegicus 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate assay at Rattus norvegicus 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.12 ? x * 17000, SDS-PAGE Rattus norvegicus
4.2.3.12 tetramer 4 * 17000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.12 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.12 6.5 7
-
 Rattus norvegicus
4.2.3.12 7.4
-
 assay at Rattus norvegicus