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Literature summary extracted from

  • Miyazaki, J.H.; Croteau, R.
    Immobilization of cyclase enzymes for the production of monoterpenes and sesquiterpenes (1990), Enzyme Microb. Technol., 12, 841-845.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.3.10 immobilized enzyme form is markedly more stable than the free form Anethum foeniculum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.10 additional information not inhibited by N-acetylsuccinimide, up to 5 mM Anethum foeniculum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.10 geranyl diphosphate Anethum foeniculum biosynthesis of monoterpenes (-)-endo-fenchol + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.10 Anethum foeniculum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.10 partial Anethum foeniculum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.10 leaf
-
Anethum foeniculum
-

Storage Stability

EC Number Storage Stability Organism
4.2.3.10 4°C, immobilized enzyme on CH-activated Sepharose, 1 month, retains over 30% of the original activity, the activity of the free enzyme is negligible Anethum foeniculum
4.2.3.10 4°C, immobilized enzyme on CH-activated Sepharose, in presence of 1 mM sodium diphosphate as substrate analog, 1 month, retains over 60% of the original activity Anethum foeniculum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.10 geranyl diphosphate
-
Anethum foeniculum (-)-endo-fenchol + diphosphate
-
?
4.2.3.10 geranyl diphosphate biosynthesis of monoterpenes Anethum foeniculum (-)-endo-fenchol + diphosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.10 30
-
assay at Anethum foeniculum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.10 additional information
-
no significant difference in the kinetics of inactivation up to 50°C of free and immobilized enzyme Anethum foeniculum