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Literature summary extracted from

  • Kollmann-Koch, A.; Eggerer, H.
    Nicotinic acid metabolism. Dimethylmaleate hydratase (1984), Hoppe-Seyler's Z. Physiol. Chem., 365, 847-857.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.85 unstable during purification, probably because of dissociation into inactive protein compounds Eubacterium barkeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.85 5,5'-dithiobis(2-nitrobenzoate) 1 mM: 97% inhibition Eubacterium barkeri
4.2.1.85 EDTA 1-5 mM: 70% loss of activity Eubacterium barkeri
4.2.1.85 additional information 10 min, 25°C, 2 mM iodoacetamide or iodoacetate: no influence Eubacterium barkeri
4.2.1.85 N-ethylmaleimide 1 mM: 93% inhibition Eubacterium barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.85 3.1
-
dimethylmaleate 35°C, pH 7 Eubacterium barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.85 Fe2+ required Eubacterium barkeri
4.2.1.85 additional information Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+ Eubacterium barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.85 153000
-
gel filtration Eubacterium barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.85 dimethylmaleate + H2O Eubacterium barkeri
-
(2R,3S)-2,3-dimethylmalate
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.85 Eubacterium barkeri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.85 oxygen-sensitive Eubacterium barkeri

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.85 ATCC 25849, partial Eubacterium barkeri

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.2.1.85 nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions Eubacterium barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.85 0.14
-
-
Eubacterium barkeri

Storage Stability

EC Number Storage Stability Organism
4.2.1.85 -20°C, 45% loss of activity within 1 day Eubacterium barkeri
4.2.1.85 4°C, 85% loss of activity within 1 day Eubacterium barkeri
4.2.1.85 liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0 Eubacterium barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.85 dimethylmaleate + H2O
-
Eubacterium barkeri (2R,3S)-2,3-dimethylmalate
-
r
4.2.1.85 dimethylmaleate + H2O equilibrium 70% in favour of 2,3-dimethylmalate, dimethylfumarate is no substrate Eubacterium barkeri (2R,3S)-2,3-dimethylmalate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.85 35
-
assay at Eubacterium barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.85 6.5 9 broad Eubacterium barkeri