Literature summary extracted from
Kollmann-Koch, A.; Eggerer, H.
Nicotinic acid metabolism. Dimethylmaleate hydratase (1984), Hoppe-Seyler's Z. Physiol. Chem., 365, 847-857.
General Stability
EC Number |
General Stability |
Organism |
---|
4.2.1.85 |
unstable during purification, probably because of dissociation into inactive protein compounds |
Eubacterium barkeri |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.1.85 |
5,5'-dithiobis(2-nitrobenzoate) |
1 mM: 97% inhibition |
Eubacterium barkeri |
|
4.2.1.85 |
EDTA |
1-5 mM: 70% loss of activity |
Eubacterium barkeri |
|
4.2.1.85 |
additional information |
10 min, 25°C, 2 mM iodoacetamide or iodoacetate: no influence |
Eubacterium barkeri |
|
4.2.1.85 |
N-ethylmaleimide |
1 mM: 93% inhibition |
Eubacterium barkeri |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.2.1.85 |
3.1 |
- |
dimethylmaleate |
35°C, pH 7 |
Eubacterium barkeri |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.1.85 |
Fe2+ |
required |
Eubacterium barkeri |
|
4.2.1.85 |
additional information |
Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+ |
Eubacterium barkeri |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.2.1.85 |
153000 |
- |
gel filtration |
Eubacterium barkeri |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.1.85 |
dimethylmaleate + H2O |
Eubacterium barkeri |
- |
(2R,3S)-2,3-dimethylmalate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.85 |
Eubacterium barkeri |
- |
- |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
4.2.1.85 |
oxygen-sensitive |
Eubacterium barkeri |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.1.85 |
ATCC 25849, partial |
Eubacterium barkeri |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
4.2.1.85 |
nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions |
Eubacterium barkeri |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.2.1.85 |
0.14 |
- |
- |
Eubacterium barkeri |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
4.2.1.85 |
-20°C, 45% loss of activity within 1 day |
Eubacterium barkeri |
4.2.1.85 |
4°C, 85% loss of activity within 1 day |
Eubacterium barkeri |
4.2.1.85 |
liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0 |
Eubacterium barkeri |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.1.85 |
dimethylmaleate + H2O |
- |
Eubacterium barkeri |
(2R,3S)-2,3-dimethylmalate |
- |
r |
|
4.2.1.85 |
dimethylmaleate + H2O |
equilibrium 70% in favour of 2,3-dimethylmalate, dimethylfumarate is no substrate |
Eubacterium barkeri |
(2R,3S)-2,3-dimethylmalate |
- |
r |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.2.1.85 |
35 |
- |
assay at |
Eubacterium barkeri |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.1.85 |
6.5 |
9 |
broad |
Eubacterium barkeri |