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Literature summary extracted from

  • Duran, R.; Chion, C.K.N.C.K.; Bigey, F.; Arnaud, A.; Galzy, P.
    The N-terminal amino acid sequences of Brevibacterium sp. R312 nitrile hydratase (1992), J. Basic Microbiol., 32, 13-19.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 24000
-
 x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
4.2.1.84 26000
-
 x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
4.2.1.84 26000
-
 x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
4.2.1.84 27000
-
 x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
4.2.1.84 27000
-
 x * 27000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
4.2.1.84 27500
-
 x * 27000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
4.2.1.84 28500
-
 x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
4.2.1.84 29000
-
 x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
4.2.1.84 29000
-
 x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
4.2.1.84 32000
-
 x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
4.2.1.84 52000
-
-
 Rhodococcus sp.
4.2.1.84 61400
-
-
 Corynebacterium sp.
4.2.1.84 70000
-
 N774 strain Rhodococcus sp.
4.2.1.84 90000 94000 gel filtration Brevibacterium sp.
4.2.1.84 100000
-
-
 Pseudomonas chlororaphis
4.2.1.84 278000
-
 strain 7 Rhodococcus sp.
4.2.1.84 420000
-
-
 Arthrobacter sp.
4.2.1.84 505000 530000
-
 Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Arthrobacter sp.
-
-
-
4.2.1.84 Brevibacterium sp.
-
-
-
4.2.1.84 Brevibacterium sp. R312
-
-
-
4.2.1.84 Corynebacterium sp.
-
-
-
4.2.1.84 Pseudomonas chlororaphis
-
-
-
4.2.1.84 Rhodococcus rhodochrous
-
-
-
4.2.1.84 Rhodococcus rhodochrous J1
-
-
-
4.2.1.84 Rhodococcus sp.
-
-
-
4.2.1.84 Rhodococcus sp. 7
-
-
-
4.2.1.84 Rhodococcus sp. N-774
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 ammonium sulfate fractionation and chromatography on Q-Sepharose, phenyl-Sepharose and Sephadex G150 Brevibacterium sp.
4.2.1.84 ammonium sulfate fractionation, chromatography on DEAE-Sephacel and phenyl-Sepharose CL-4B and gel filtration, R312 strain, 10.2fold purified Brevibacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
-
 Rhodococcus rhodochrous
4.2.1.84 additional information
-
-
 Corynebacterium sp.
4.2.1.84 additional information
-
-
 Rhodococcus sp.
4.2.1.84 additional information
-
-
 Brevibacterium sp.
4.2.1.84 additional information
-
-
 Pseudomonas chlororaphis
4.2.1.84 2000
-
 purified enzyme Brevibacterium sp.

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
4.2.1.84 ? x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
4.2.1.84 ? x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
4.2.1.84 ? x * 27000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
4.2.1.84 ? x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
4.2.1.84 tetramer
-
 Rhodococcus rhodochrous
4.2.1.84 tetramer
-
 Brevibacterium sp.
4.2.1.84 tetramer
-
 Pseudomonas chlororaphis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.84 Brevibacterium sp. polyacrylamide isoelectrofocusing
-
 5.75