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Literature summary extracted from

  • Asano, Y.; Fujishiro, K.; Tani, Y.; Yamada, H.
    Aliphatic nitrile hydratase from Arthrobacter sp. J-1. Purification and characterization (1982), Agric. Biol. Chem., 46, 1165-1174.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.84 glycine-NaOH activates Arthrobacter sp.

General Stability

EC Number General Stability Organism
4.2.1.84 potassium phosphate buffer has a negative effect on stability Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 Ag+ complete inhibition at 1 mM of AgNO3 Arthrobacter sp.
4.2.1.84 BaCl2 weak inhibition Arthrobacter sp.
4.2.1.84 glutaronitrile
-
 Arthrobacter sp.
4.2.1.84 Hg2+ complete inhibition at 0.01 mM Hg2+ Arthrobacter sp.
4.2.1.84 iodoacetic acid
-
 Arthrobacter sp.
4.2.1.84 Isobutyronitrile
-
 Arthrobacter sp.
4.2.1.84 KCN complete inhibition at 0.1 mM, competitive with acetonitrile as substrate Arthrobacter sp.
4.2.1.84 p-chloromercuribenzoate strong inhibition at 1 mM Arthrobacter sp.
4.2.1.84 sulfhydryl reagents
-
 Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 0.88
-
 acrylonitrile pH 7, 30°C Arthrobacter sp.
4.2.1.84 1.9
-
 Propionitrile pH 7, 30°C Arthrobacter sp.
4.2.1.84 5.78
-
 acetonitrile pH 7, 30°C Arthrobacter sp.
4.2.1.84 8.77
-
 Methacrylonitrile pH 7, 30°C Arthrobacter sp.
4.2.1.84 10.8
-
 n-butyronitrile pH 7, 30°C Arthrobacter sp.
4.2.1.84 10.9
-
 Chloroacetonitrile pH 7, 30°C Arthrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.84 intracellular
-
 Arthrobacter sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 24000
-
 x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
4.2.1.84 27000
-
 x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
4.2.1.84 420000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Arthrobacter sp.
-
-
-
4.2.1.84 Arthrobacter sp. J-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 to homogeneity, using several chromatographic steps, including DEAE, hydroxyapatite, and Sephadex G-200 column chromatography Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 9.17
-
-
 Arthrobacter sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.84 -20°C, pH 7.0, 50% glycerol, stable for at least 40 days, 40% loss of activity after 6 months Arthrobacter sp.
4.2.1.84 0°C, pH 7.0, 50% loss of activity after 40 days Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acetonitrile + H2O
-
 Arthrobacter sp. acetamide
-
 ir
4.2.1.84 acetonitrile + H2O
-
 Arthrobacter sp. J-1 acetamide
-
 ir
4.2.1.84 acrylonitrile + H2O
-
 Arthrobacter sp. 2-propenoic acid amide i.e. acrylic acid amide r
4.2.1.84 chloroacetonitrile + H2O
-
 Arthrobacter sp. chloroacetamide
-
 ir
4.2.1.84 chloroacetonitrile + H2O
-
 Arthrobacter sp. J-1 chloroacetamide
-
 ir
4.2.1.84 hydroxyacetonitrile + H2O
-
 Arthrobacter sp. hydroxyacetamide
-
 r
4.2.1.84 methacrylonitrile + H2O
-
 Arthrobacter sp. methylacrylic acid amide
-
 r
4.2.1.84 additional information aliphatic nitriles with more than 5 carbons and aromatic nitriles cannot act as substrate Arthrobacter sp. ?
-
 ?
4.2.1.84 additional information aliphatic nitriles with more than 5 carbons and aromatic nitriles cannot act as substrate Arthrobacter sp. J-1 ?
-
 ?
4.2.1.84 n-butyronitrile + H2O
-
 Arthrobacter sp. n-butyric acid amide
-
 r
4.2.1.84 n-butyronitrile + H2O
-
 Arthrobacter sp. J-1 n-butyric acid amide
-
 r
4.2.1.84 propionitrile + H2O
-
 Arthrobacter sp. propionic acid amide i.e. propionamide r
4.2.1.84 propionitrile + H2O
-
 Arthrobacter sp. J-1 propionic acid amide i.e. propionamide r

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 35
-
-
 Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 35
-
 10 min, 20% loss of activity Arthrobacter sp.
4.2.1.84 35
-
 1 hour, 10 mM methacrylonitrile stable up to this temperature Arthrobacter sp.
4.2.1.84 55
-
 10 min, nearly complete inactivation Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7 7.2
-
 Arthrobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.84 0.015
-
 KCN pH 7, 30°C Arthrobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.84 Arthrobacter sp.
-
-
 3.6