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Literature summary extracted from

  • Kunz, D.A.; Ribbons, D.W.; Chapman, P.J.
    Metabolism of allylglycine and cis-crotylglycine by Pseudomonas putida (arvilla) mt-2 habouring a TOL plasmid (1981), J. Bacteriol., 148, 72-82.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.80 Pseudomonas putida
-
 TOL plasmid-haboring
-
4.2.1.80 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
 TOL plasmid-haboring
-
4.2.1.80 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.80
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.80 0.33 0.54 cell-free extracts from succinate-grown cells, strain-dependent Pseudomonas putida
4.2.1.80 5.2
-
 cell-free extracts from DL-allylglycerine grown cells Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.80 2-keto-cis-4-hexenoic acid 50% the rate of 4-hydroxy-2-oxopentanoate, higher activity towards cis than towards trans-isomer Pseudomonas putida ?
-
 ?
4.2.1.80 2-keto-cis-4-hexenoic acid 50% the rate of 4-hydroxy-2-oxopentanoate, higher activity towards cis than towards trans-isomer Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
4.2.1.80 4-hydroxy-2-oxopentanoate
-
 Pseudomonas sp. 2-oxopent-4-enoate + H2O
-
 ?
4.2.1.80 4-hydroxy-2-oxopentanoate
-
 Pseudomonas putida 2-oxopent-4-enoate + H2O
-
 ?
4.2.1.80 4-hydroxy-2-oxopentanoate
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-oxopent-4-enoate + H2O
-
 ?