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Literature summary extracted from

  • Gonzales, B.; Vicuna, R.
    Benzaldehyde lyase, a novel thiamine PPi-requiring enzyme, from Pseudomonas fluorescens biovar I (1989), J. Bacteriol., 171, 2401-2405.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.38 2-Acetoxy-1,2-bis(4'-methoxyphenyl)-ethanone 0.2 mM: 50% inhibition Pseudomonas fluorescens
4.1.2.38 Desoxyanisoin 0.4 mM: 50% inhibition Pseudomonas fluorescens
4.1.2.38 desoxybenzoin 0.9 mM: 50% inhibition Pseudomonas fluorescens
4.1.2.38 additional information benzoylformic acid at 0.9 mM, pyruvic acid at 10 mM, acetoin at 10 mM, N-ethylmaleimide at 20 mM, dithiothreitol at 5 mM are no inhibitors Pseudomonas fluorescens
4.1.2.38 thiamine diphosphate more than 0.5 mM: inhibition, requirement for 0.01 mM Pseudomonas fluorescens
4.1.2.38 Urea 4 mM: inactivation Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.38 0.009
-
 Benzoin 37°C, pH 8 Pseudomonas fluorescens
4.1.2.38 0.0325
-
 Anisoin 37°C, pH 8 Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Ba2+ less effective than Mg2+ or Mn2+ or Ca2+ Pseudomonas fluorescens
4.1.2.38 Ca2+ 1 mM, absolute requirement of a divalent cation, may be substituted by Mn2+ or Mg2+ Pseudomonas fluorescens
4.1.2.38 Cu2+ less effective than Mg2+ or Mn2+ or Ca2+ Pseudomonas fluorescens
4.1.2.38 Mg2+ 1 mM, absolute requirement for a divalent cation, may be substituted by Mn2+ or Ca2+ Pseudomonas fluorescens
4.1.2.38 Mn2+ 1 mM, absolute requirement for a divalent cation, may be substituted by Mg2+ or Ca2+ Pseudomonas fluorescens
4.1.2.38 additional information Co2+, Zn2+, Ni2+, Fe2+, Al2+ at 1 mM are ineffective. KCl, NaCl, NH4Cl at 1-160 mM: no influence Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.38 53000
-
 x * 53000, SDS-PAGE Pseudomonas fluorescens
4.1.2.38 80000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens
-
 strain biovar I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.38 strain biovar I, partial Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.38 11690
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
4.1.2.38 -20°C, purified enzyme, stable for at least 2 months Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 anisoin
-
 Pseudomonas fluorescens 4-methoxybenzaldehyde + 4-methoxybenzaldehyde
-
 ?
4.1.2.38 benzoin
-
 Pseudomonas fluorescens benzaldehyde + benzaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.38 ? x * 53000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.38 37
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.38 7.5 8.5 broad Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.38 6 8.5 no activity at pH 6 and below, maximal activity at pH 7.5-8.5 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate absolute requirement, 0.01 mM optimal, more than 0.5 mM: inhibition Pseudomonas fluorescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.38 0.2
-
 2-Acetoxy-1,2-bis(4'-methoxyphenyl)-ethanone 37°C, pH 8 Pseudomonas fluorescens
4.1.2.38 0.4
-
 Desoxyanisoin 37°C, pH 8 Pseudomonas fluorescens
4.1.2.38 0.9
-
 desoxybenzoin 37°C, pH 8 Pseudomonas fluorescens