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Literature summary extracted from

  • Mörsberger, F.M.; Müller, R.; Otto, M.K.; Lingens, F.; Kulbe, K.D.
    Purification and characterization of 2-halocarboxylic acid dehalogenase II from Pseudomonas spec. CBS 3 (1991), Biol. Chem. Hoppe-Seyler, 372, 915-922.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.2 FeSO4 above 10 mM Pseudomonas sp.
3.8.1.2 mercury(II)-acetate
-
 Pseudomonas sp.
3.8.1.2 MnSO4 above 10 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 0.57
-
 L-2-chloropropionate pH 9.5, 37°C Pseudomonas sp.
3.8.1.2 1.6
-
 Monochloroacetate pH 9.5, 37°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 29000
-
 2 * 29000 SDS-PAGE Pseudomonas sp.
3.8.1.2 64000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.8.1.2 dithiothreitol increases enzyme activity slight Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas sp.
-
-
-
3.8.1.2 Pseudomonas sp. CBS3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.2 13.8
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.8.1.2 50 mM Tris sulfate, pH 7.5 + 0.01% NaN3 at 25°C or -30°C, 30 days with remaining 60% activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted Pseudomonas sp. CBS3 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 2-bromopropionic acid + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas sp. CBS3 D-lactate + HCl
-
 ?
3.8.1.2 L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. D-2-hydroxypropionic acid + HCl
-
 ?
3.8.1.2 L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. CBS3 D-2-hydroxypropionic acid + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?
3.8.1.2 monobromoacetate + H2O
-
 Pseudomonas sp. CBS3 glycolate + HBr
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Pseudomonas sp. CBS3 glycolate + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 dimer 2 * 29000 SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 40 45
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 9.5
-
-
 Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.8.1.2 Pseudomonas sp. isoelectric focusing
-
 6.2