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Literature summary extracted from

  • Ordaz., E.; Garrido-Pertierra, A.; Gallego, M.; Puyet, A.
    Covalent and metal-chelate immobilization of a modified 2-haloacid dehalogenase for the enzymatic resolution of optically active chloropropionic acid (2000), Biotechnol. Prog., 16, 287-291.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.8.1.2 synthesis His-tagged L-2-haloacid dehalogenase subtype immobilized for production of D-lactate and D-chloropropionic acid Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expression in Escherichia coli Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.2 additional information
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 additional information
-
 additional information covalently immobilized L-2-haloacid dehalogenase subtype shows KM-value of 23.3 mM Pseudomonas sp.
3.8.1.2 7.5
-
 2-monochloropropionic acid pH 8.5, 37°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 2-chloropropionate + H2O Pseudomonas sp.
-
 lactate + HCl
-
 ?
3.8.1.2 2-chloropropionate + H2O Pseudomonas sp. CBS3
-
 lactate + HCl
-
 ?
3.8.1.2 L-2-chloropropionic acid + H2O Pseudomonas sp.
-
 D-2-hydroxypropionic acid + HCl
-
 ?
3.8.1.2 L-2-chloropropionic acid + H2O Pseudomonas sp. CBS3
-
 D-2-hydroxypropionic acid + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp. CBS3
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas sp.
-
-
-
3.8.1.2 Pseudomonas sp. CBS3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. CBS3 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Pseudomonas sp. lactate + HCl
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Pseudomonas sp. CBS3 lactate + HCl
-
 ?
3.8.1.2 L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. D-2-hydroxypropionic acid + HCl
-
 ?
3.8.1.2 L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. CBS3 D-2-hydroxypropionic acid + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. CBS3 D-2-hydroxyalkanoic acid + halide
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.2 additional information
-
 enzyme covalently immobilized is stable between 30°C and 85°C Pseudomonas sp.
3.8.1.2 25 70
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.8.1.2 7.5 11
-
 Pseudomonas sp.