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Literature summary extracted from

  • Liu, J.Q.; Kurihara, T.; Nardi-Dei, V.; Okamura, T.; Esaki, N.; Soda, K.
    Overexpression and feasible purification of a thermostable L-2-halo acid dehalogenase of Pseudomonas sp. YL (1995), Biodegradation, 6, 223-227.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.8.1.2 synthesis L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expression in Escherichia coli Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 27000
-
 2 * 27000, SDS-PAGE Pseudomonas sp.
3.8.1.2 54000
-
 SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 2-chloropropionate + H2O Pseudomonas sp.
-
 lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monobromoacetate + H2O Pseudomonas sp.
-
 glycolate + HBr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.2 118
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Pseudomonas sp. lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 dimer 2 * 27000, SDS-PAGE Pseudomonas sp.