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Literature summary extracted from

  • Liu, J.Q.; Kurihara, T.; Hasan, A.K.M.Q.; Nardi-Dei, V.; Koshikawa, H.; Esaki, N.; Soda, K.
    Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL (1994), Appl. Environ. Microbiol., 60, 2389-2393.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.8.1.2 synthesis L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.2 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
 Pseudomonas sp.
3.8.1.2 HgSO4
-
 Pseudomonas sp.
3.8.1.2 iodoacetamide
-
 Pseudomonas sp.
3.8.1.2 N-ethylmaleimide
-
 Pseudomonas sp.
3.8.1.2 p-chloromercuriphenylsulfonic acid
-
 Pseudomonas sp.
3.8.1.2 Woodward reagent K
-
 Pseudomonas sp.
3.8.1.10 Hg2+ HgSO4 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 0.37
-
 L-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
3.8.1.2 1.1
-
 Monochloroacetate pH 9.5, 30°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 27000
-
 2 * 27000, SDS-PAGE Pseudomonas sp.
3.8.1.2 54000
-
 gel filtration Pseudomonas sp.
3.8.1.10 36000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 2-chloropropionate + H2O Pseudomonas sp.
-
 lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monobromoacetate + H2O Pseudomonas sp.
-
 glycolate + HBr
-
 ?
3.8.1.2 monochloroacetate + H2O Pseudomonas sp.
-
 glycolate + HCl
-
 ?
3.8.1.2 monoiodoacetate + H2O Pseudomonas sp.
-
 glycolate + HI
-
 ?
3.8.1.10 (R)-2-haloacid + H2O Pseudomonas sp.
-
 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O Pseudomonas sp.
-
 (R)-2-hydroxyacid + halide
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.8.1.2 n-heptane retaining 3.3% activity of that in water with substrate 2-chloropropionate Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas sp.
-
-
-
3.8.1.10 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Pseudomonas sp.
3.8.1.10
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.2 120
-
-
 Pseudomonas sp.
3.8.1.10 30
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.8.1.2 -20°C, pH 7.5 + 50% glycerol, 6 months, strain YL Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 2,2-dichloropropionate + H2O
-
 Pseudomonas sp. propionate + HCl
-
 ?
3.8.1.2 2-bromohexadecanoate + H2O
-
 Pseudomonas sp. D-2-hydroxyhexadecanoate + HBr
-
 ?
3.8.1.2 2-chloroacrylate + H2O
-
 Pseudomonas sp. 2-hydroxyacrylate + HCl
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Pseudomonas sp. lactate + HCl
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
3.8.1.2 monoiodoacetate + H2O
-
 Pseudomonas sp. glycolate + HI
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 D-2-monochloropropionate + H2O
-
 Pseudomonas sp. L-lactate + HCl
-
 ?
3.8.1.10 L-2-monochloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
3.8.1.10 monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?
3.8.1.10 monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
3.8.1.10 monoiodoacetate + H2O
-
 Pseudomonas sp. glycolate + HI
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 dimer 2 * 27000, SDS-PAGE Pseudomonas sp.
3.8.1.10 monomer 1 * 36000 SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 65
-
-
 Pseudomonas sp.
3.8.1.10 45
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.2 60
-
 enzyme retains its full activity for 30 min Pseudomonas sp.
3.8.1.10 60
-
 30 min 80% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 9.5
-
-
 Pseudomonas sp.
3.8.1.10 10.5
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.8.1.2 4.5 10.5 at 30°C for 30 min Pseudomonas sp.