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Literature summary extracted from

  • Motosugi, K; Esahi, N.; Soda, K.
    Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid (1984), Biotechnol. Bioeng., 26, 805-806.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.8.1.2 synthesis production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry Pseudomonas sp.
3.8.1.2 synthesis production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.10 1.1
-
 L-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
3.8.1.10 3.2
-
 DL-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
3.8.1.10 4.8
-
 D-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas putida
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas sp. 113
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas putida
-
-
-
3.8.1.2 Pseudomonas sp.
-
-
-
3.8.1.2 Pseudomonas sp. 113
-
-
-
3.8.1.10 Pseudomonas putida
-
-
-
3.8.1.10 Pseudomonas sp.
-
-
-
3.8.1.10 Pseudomonas sp. 113
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas putida (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas sp. 113 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas putida D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. 113 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.10 D-2-monochloropropionate + H2O
-
 Pseudomonas sp. L-lactate + HCl
-
 ?
3.8.1.10 D-2-monochloropropionate + H2O
-
 Pseudomonas sp. 113 L-lactate + HCl
-
 ?
3.8.1.10 DL-2-chloropropionate + H2O
-
 Pseudomonas sp. DL-lactate + HCl
-
 ?
3.8.1.10 DL-2-chloropropionate + H2O
-
 Pseudomonas sp. 113 DL-lactate + HCl
-
 ?

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.8.1.10 Pseudomonas sp. isoelectric focusing
-
 4.9