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Literature summary extracted from

  • Huffman, J.L.; Li, H.; White, R.H.; Tainer, J.A.
    Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii (2003), J. Mol. Biol., 331, 885-896.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.13 expression in Escherichia coli Methanocaldococcus jannaschii
3.6.1.23 expressed in Escherichia coli BL21 Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.13 apoenzyme in complex with dCTP and with dUTP Methanocaldococcus jannaschii
3.5.4.30 apo form complexed with dCTP and dUTP, hanging drop vapor diffusion method Methanocaldococcus jannaschii
3.6.1.23 hanging drop vapor-diffusion method Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 dTTP competitive feedback inhibition Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 additional information
-
 additional information lower affinity for dUTP than strict dUTPases Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.30 77900
-
 enzyme with addition of six histidines at the N-terminus, gel filtration Methanocaldococcus jannaschii
3.6.1.23 136000
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.30 dCTP + H2O Methanocaldococcus jannaschii first step in conversion of dCTP to dTTP dUMP + diphosphate + NH3
-
 ?
3.6.1.23 dUTP + H2O Methanocaldococcus jannaschii removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.13 Methanocaldococcus jannaschii Q57872 bifunctional enzyme dCTP deaminase-dUTP nucleotidohydrolase
-
3.5.4.30 Methanocaldococcus jannaschii Q57872
-
-
3.6.1.23 Methanocaldococcus jannaschii Q57872
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.30
-
 Methanocaldococcus jannaschii
3.6.1.23 recombinant protein Methanocaldococcus jannaschii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.13 dCTP + H2O = dUTP + NH3 mechanism of bifunctional enzyme activity and feedback inhibition by dTTP Methanocaldococcus jannaschii
a

Storage Stability

EC Number Storage Stability Organism
3.6.1.23 -70°C, 50 mM HEPES, pH 7.2, 150 mM NaCl, 2 mM dithiothreitol Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.30 dCTP + H2O
-
 Methanocaldococcus jannaschii dUMP + diphosphate + NH3
-
 ?
3.5.4.30 dCTP + H2O first step in conversion of dCTP to dTTP Methanocaldococcus jannaschii dUMP + diphosphate + NH3
-
 ?
3.5.4.30 additional information enzyme has also dUTP diphosphatase activity, dCTP deamination and dUTP diphosphatase activities occur independently at the same active site Methanocaldococcus jannaschii ?
-
 ?
3.6.1.23 dTTP + H2O
-
 Methanocaldococcus jannaschii dTMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Methanocaldococcus jannaschii dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP Methanocaldococcus jannaschii dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.13 hexamer crystallization data Methanocaldococcus jannaschii
3.5.4.30 hexamer two trimers can assemble into a hexamer by stacking on top of each other Methanocaldococcus jannaschii
3.5.4.30 trimer two trimers can assemble into a hexamer by stacking on top of each other Methanocaldococcus jannaschii
3.6.1.23 hexamer two trimers assemble into a hexamer, crystal structure analysis Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 DCD-DUT catalyzes the deamination of dCTP and the dephosphorylation of dUTP without releasing an intermediate Methanocaldococcus jannaschii