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Literature summary extracted from

  • Aranishi, F.; Ogata, H.; Hara, K.; Osatomi, K.; Ishihara, T.M.
    Purification and characterization of cathepsin L from hepatopancreas of carp Cyprinus carpio (1997), Comp. Biochem. Physiol. B, 118, 531-537.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 Cys activates Rattus norvegicus
3.4.22.15 Cys activates Cyprinus carpio

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 1,10-phenanthroline
-
 Cyprinus carpio
3.4.22.15 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Cyprinus carpio
3.4.22.15 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Rattus norvegicus
3.4.22.15 AgNO3
-
 Cyprinus carpio
3.4.22.15 antipain
-
 Cyprinus carpio
3.4.22.15 CdCl2
-
 Cyprinus carpio
3.4.22.15 chymostatin
-
 Cyprinus carpio
3.4.22.15 CoCl2 partial Cyprinus carpio
3.4.22.15 CuCl2
-
 Cyprinus carpio
3.4.22.15 FeCl3
-
 Cyprinus carpio
3.4.22.15 Hg2+ HgCl2 Cyprinus carpio
3.4.22.15 Hg2+
-
 Rattus norvegicus
3.4.22.15 leupeptin
-
 Cyprinus carpio
3.4.22.15 leupeptin
-
 Rattus norvegicus
3.4.22.15 PCMB
-
 Cyprinus carpio
3.4.22.15 PCMB
-
 Rattus norvegicus
3.4.22.15 SnCl2 < partial Cyprinus carpio
3.4.22.15 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
 Cyprinus carpio
3.4.22.15 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane i.e. E-64, in vivo, the inhibitor is transported to the liver cytosol in the free form in the blood and permeated into the lysosomes, where it binds to, and inactivates the enzyme Rattus norvegicus
3.4.22.15 Zn(CH3COO)2
-
 Cyprinus carpio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.15 0.03416
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio
3.4.22.15 0.04371
-
 Pro-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio
3.4.22.15 0.1417
-
 tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide
-
 Cyprinus carpio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.15 lysosome
-
 Rattus norvegicus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 24000
-
 x * 24000 + x * 30000, SDS-PAGE under reducing conditions Cyprinus carpio
3.4.22.15 30000
-
 gel filtration Cyprinus carpio

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Cyprinus carpio
-
-
-
3.4.22.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15
-
 Cyprinus carpio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.15 hepatopancreas
-
 Cyprinus carpio
-
3.4.22.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.15 additional information
-
-
 Cyprinus carpio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.15 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Cyprinus carpio ?
-
 ?
3.4.22.15 Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Cyprinus carpio ?
-
 ?
3.4.22.15 tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O
-
 Cyprinus carpio ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ?
-
 Rattus norvegicus
3.4.22.15 ? x * 24000 + x * 30000, SDS-PAGE under reducing conditions Cyprinus carpio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.15 50
-
 hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Cyprinus carpio

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.15 30
-
 less than half maximal activity below 30°C and above 60°C Cyprinus carpio

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.15 40
-
 stable below Cyprinus carpio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.15 1.52
-
 Pro-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio
3.4.22.15 1.67
-
 tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide
-
 Cyprinus carpio
3.4.22.15 9.5
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Cyprinus carpio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 5.5 6 hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Cyprinus carpio

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.15 5 6.5 stable Cyprinus carpio