Literature summary extracted from

Zong, Y.; Bice, T.W.; Ton-That, H.; Schneewind, O.; Narayana, S.V.L.
Crystal structures of Staphylococcus aureus sortase A and its substrate somplex (2004), J. Biol. Chem., 279, 31383-31389.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.4.22.70	crystals are grown by the hanging-drop technique with a protein concentration of 50 mg/ml in 25 mM MES buffer, pH 6.35. The crystallization conditions include 3.2 M ammonium sulfate, 0.1 M NaCl, and trace amounts of ethylene glycol. Crystal structure of native SrtA, of an active-site mutant of SrtA, and of the mutant SrtA complexed with its substrate LPETG peptide	Staphylococcus aureus

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.22.70	DELTA N59	trucation of N-terminal 59 amino acids does not affect the activity of the enzyme	Staphylococcus aureus
3.4.22.70	DELTA N59	enhanced solubility of the mutant helps the crystallization	Staphylococcus aureus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.70	Staphylococcus aureus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.22.70	-	Staphylococcus aureus

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Release 2023.1 (January 2023)