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Literature summary extracted from

  • Chandler, C.J.; Wang, T.T.Y.; Halsted, C.H.
    Pteroylpolyglutamate hydrolase from human jejunal brush borders. Purification and characterization (1986), J. Biol. Chem., 261, 928-933.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.9 NaF
-
 Homo sapiens
3.4.19.9 p-Aminobenzoate 0.1 mM: no inhibition, 30% inhibition at 1.0 mM Homo sapiens
3.4.19.9 substituted and reduced pteroylglutamate 0.1 mM: not, 30% inhibition at 1.0 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.9 0.00055
-
 Pteroyltriglutamate
-
 Homo sapiens
3.4.19.9 0.0006
-
 Pteroylheptaglutamate
-
 Homo sapiens
3.4.19.9 0.0006
-
 pteroyldiglutamate
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.19.9 Ca2+ slight activation Homo sapiens
3.4.19.9 Ca2+ activation Homo sapiens
3.4.19.9 Cd2+ slight activation Homo sapiens
3.4.19.9 Fe2+ slight activation Homo sapiens
3.4.19.9 Mg2+ slight activation Homo sapiens
3.4.19.9 Mg2+ activation Homo sapiens
3.4.19.9 Mn2+ slight activation Homo sapiens
3.4.19.9 Mn2+ activation Homo sapiens
3.4.19.9 Ni2+ slight activation Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.9 115000
-
 x * 145000 + x * 115000, SDS/urea PAGE Homo sapiens
3.4.19.9 145000
-
 x * 145000 + x * 115000, SDS/urea PAGE Homo sapiens
3.4.19.9 700000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.9 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.9
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.9 jejunum mucosal brush-border Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.9 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.9 pteroyldiglutamate + H2O
-
 Homo sapiens ?
-
 ?
3.4.19.9 pteroylheptaglutamate + H2O
-
 Homo sapiens ?
-
 ?
3.4.19.9 pteroyltriglutamate + H2O
-
 Homo sapiens ?
-
 ?
3.4.19.9 pteroyltriglutamate + H2O
-
 Homo sapiens pteroylglutamate + glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.9 ? x * 145000 + x * 115000, SDS/urea PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.9 60
-
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.19.9 30
-
 about 25% of activity maximum at 30°C and 80°C Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.9 65
-
 up to, for at least 90 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.9 5.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.19.9 4 7 about 45% of activity maximum at pH 4 and pH 7 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.19.9 6.5
-
 stable at pH 6.5 and above, 90 min Homo sapiens