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Literature summary extracted from
- Purification and properties of carboxypeptidase G2 from Pseudomonas sp. strain RS-16. Use of a novel triazine dye affinity method (1985), Eur. J. Biochem., 148, 447-453.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.11 | 0.004 | - |
folate | - |
Pseudomonas sp. |  |
| 3.4.17.11 | 0.008 | - |
methotrexate | - |
Pseudomonas sp. | |
| 3.4.17.11 | 0.034 | - |
5-methyltetrahydrofolate | - |
Pseudomonas sp. | |
| 3.4.17.11 | 0.12 | - |
5-formyltetrahydrofolate | - |
Pseudomonas sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.11 | Zn2+ | contains 4 atoms of zinc per enzyme molecule, which are required for full activity | Pseudomonas sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.11 | 41800 | - |
2 * 41800, SDS-PAGE | Pseudomonas sp. |
| 3.4.17.11 | 83000 | - |
gel filtration | Pseudomonas sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.11 | Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.11 | large scale | Pseudomonas sp. |
| 3.4.17.11 | strain RS-16 | Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.11 | 215 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.11 | 5-formyltetrahydrofolic acid + H2O | - |
Pseudomonas sp. | 5-formylpteroate + Glu | - |
? | |
| 3.4.17.11 | 5-methyltetrahydrofolate + H2O | - |
Pseudomonas sp. | 5-methylpteroate + Glu | - |
? | |
| 3.4.17.11 | folic acid + H2O | - |
Pseudomonas sp. | pteroic acid + glutamic acid | - |
? | |
| 3.4.17.11 | methotrexate + H2O | - |
Pseudomonas sp. | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.11 | dimer | 2 * 41800, SDS-PAGE | Pseudomonas sp. |
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