Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Asano, Y.; Nakazawa, A.; Kato, Y.; Kondo, K.
    Properties of a novel D-stereospecific aminopeptidase from Ochrobactrum anthropi (1989), J. Biol. Chem., 264, 14233-14239.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.19 additional information thiol-dependent Brucella anthropi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.19 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Brucella anthropi
3.4.11.19 Ag+
-
 Brucella anthropi
3.4.11.19 ampicillin
-
 Brucella anthropi
3.4.11.19 Ca2+ 10 mM Brucella anthropi
3.4.11.19 Cd2+ 10 mM Brucella anthropi
3.4.11.19 Cu2+ 10 mM Brucella anthropi
3.4.11.19 Hg2+
-
 Brucella anthropi
3.4.11.19 hydroxylamine
-
 Brucella anthropi
3.4.11.19 additional information probably has serine at the active centre, not: EDTA (0.2 mM) Brucella anthropi
3.4.11.19 NEM
-
 Brucella anthropi
3.4.11.19 Ni2+ 10 mM Brucella anthropi
3.4.11.19 PCMB
-
 Brucella anthropi
3.4.11.19 Zn2+ 10 mM Brucella anthropi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.19 0.32
-
 D-Ala4
-
 Brucella anthropi
3.4.11.19 0.37
-
 D-Ala-Gly-Gly
-
 Brucella anthropi
3.4.11.19 0.51
-
 D-alanine-4-nitroanilide
-
 Brucella anthropi
3.4.11.19 0.51
-
 D-Ala benzylamide
-
 Brucella anthropi
3.4.11.19 0.57
-
 D-Ala-D-Ala-D-Ala
-
 Brucella anthropi
3.4.11.19 0.65
-
 D-alanine amide
-
 Brucella anthropi
3.4.11.19 0.65
-
 D-Ala-L-Ala-L-Ala
-
 Brucella anthropi
3.4.11.19 0.73
-
 D-Ala-n-butylamide
-
 Brucella anthropi
3.4.11.19 0.98
-
 D-Ala-Gly
-
 Brucella anthropi
3.4.11.19 1.03
-
 D-Ala-L-Ala
-
 Brucella anthropi
3.4.11.19 2.27
-
 D-Ala-3-aminopentane amide
-
 Brucella anthropi
3.4.11.19 10.2
-
 D-Ala-D-Ala
-
 Brucella anthropi
3.4.11.19 18.3
-
 D-2-Aminobutyric acid amide
-
 Brucella anthropi
3.4.11.19 22.3
-
 glycine amide
-
 Brucella anthropi
3.4.11.19 27
-
 D-Serine amide
-
 Brucella anthropi
3.4.11.19 100
-
 D-threonine amide
-
 Brucella anthropi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.19 additional information not metal-dependent Brucella anthropi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.19 59000
-
 2 * 59000, SDS-PAGE Brucella anthropi
3.4.11.19 122000
-
 gel filtration Brucella anthropi

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.19 Brucella anthropi
-
 SCRC C1-38
-
3.4.11.19 Brucella anthropi SCRC C1-38
-
 SCRC C1-38
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.19
-
 Brucella anthropi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.19 additional information
-
-
 Brucella anthropi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.19 D-2-aminobutyric acid amide + H2O
-
 Brucella anthropi D-aminobutyric acid + NH3
-
 ?
3.4.11.19 D-2-aminobutyric acid amide + H2O
-
 Brucella anthropi SCRC C1-38 D-aminobutyric acid + NH3
-
 ?
3.4.11.19 D-Ala 4-nitroanilide + H2O
-
 Brucella anthropi D-Ala + 4-nitroaniline
-
 ?
3.4.11.19 D-Ala-3-aminopentane amide + H2O
-
 Brucella anthropi ?
-
 ?
3.4.11.19 D-Ala-D-Ala + H2O
-
 Brucella anthropi D-Ala
-
 ?
3.4.11.19 D-Ala-Gly + H2O
-
 Brucella anthropi D-Ala + Gly
-
 ?
3.4.11.19 D-Ala-Gly + H2O
-
 Brucella anthropi SCRC C1-38 D-Ala + Gly
-
 ?
3.4.11.19 D-Ala-Gly-Gly + H2O
-
 Brucella anthropi D-Ala + Gly-Gly
-
 ?
3.4.11.19 D-Ala-L-Ala-L-Ala + H2O
-
 Brucella anthropi D-Ala + Ala-Ala
-
 ?
3.4.11.19 D-Ala4 + H2O
-
 Brucella anthropi ?
-
 ?
3.4.11.19 D-alanine amide + H2O
-
 Brucella anthropi D-Ala + NH3
-
 ?
3.4.11.19 D-alanine amide + H2O
-
 Brucella anthropi SCRC C1-38 D-Ala + NH3
-
 ?
3.4.11.19 D-alanine benzylamide + H2O
-
 Brucella anthropi D-Ala + benzylamine
-
 ?
3.4.11.19 D-alanine n-butylamide + H2O
-
 Brucella anthropi D-Ala + n-butylamine
-
 ?
3.4.11.19 D-serine amide + H2O
-
 Brucella anthropi D-serine + NH3
-
 ?
3.4.11.19 D-serine amide + H2O
-
 Brucella anthropi SCRC C1-38 D-serine + NH3
-
 ?
3.4.11.19 D-threonine amide + H2O
-
 Brucella anthropi D-Thr + NH3
-
 ?
3.4.11.19 glycine amide + H2O
-
 Brucella anthropi Gly + NH3
-
 ?
3.4.11.19 additional information strict D-stereospecificity, the enzyme prefers peptides to amino acid arylamides or amino acid amides Brucella anthropi ?
-
 ?
3.4.11.19 additional information strict D-stereospecificity, the enzyme prefers peptides to amino acid arylamides or amino acid amides Brucella anthropi SCRC C1-38 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.19 dimer 2 * 59000, SDS-PAGE Brucella anthropi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.19 45
-
-
 Brucella anthropi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.19 30
-
 pH 7.0-10.9, 1 h, no loss of activity Brucella anthropi
3.4.11.19 45
-
 pH 8.0, 10 min, about 80% of original activity retained Brucella anthropi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.19 8.5
-
-
 Brucella anthropi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.19 7 10 30°C, 1 h, no loss of activity Brucella anthropi
3.4.11.19 8
-
 45°C, 10 min, about 80% of the original activity retained Brucella anthropi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.19 Brucella anthropi
-
-
 4.2