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Literature summary extracted from

  • Nielsen, G.M.; Bao, Y.; Roberts, G.P.; Ludden, P.W.
    Purification and characterization of an oxygen-stable form of dinitrogenase reductase-activating glycohydrolase from Rhodospirillum rubrum (1994), Biochem. J., 302, 801-806.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.2.24 Mn2+ renders the enzyme more susceptible to inactivation by dithionite in air, not Ca2+, Fe2+ or Mg2+, ADPribose protects, not ADP Rhodospirillum rubrum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.24 ADPribose
-
 Rhodospirillum rubrum
3.2.2.24 Ca2+
-
 Rhodospirillum rubrum
3.2.2.24 Dithionite in air, inactivation Rhodospirillum rubrum
3.2.2.24 H2O2 inactivation, to the same extent as dithionite in air Rhodospirillum rubrum
3.2.2.24 additional information no inhibition by ADP Rhodospirillum rubrum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.24 additional information no covalently bound inorganic cofactors, inductively coupled plasma emission analysis, detection limit for Mn2+: 0.00005 mM Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex Rhodospirillum rubrum
-
 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.24 Rhodospirillum rubrum
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.2.2.24 attempts to purify the enzyme using aerobic buffer are partly successful, aerobic DEAE-cellulose chromatography results in rapid loss of activity, 30 min stable to exposure to air in buffer containing DTT in the absence of dithionite, in its presence decrease of activity with time, bovine serum albumin does not protect Rhodospirillum rubrum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.24
-
 Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.24 40.1
-
 strain UR1, anaerobic preparation, prepared in the presence of 1 mM dithionite, pH 7.5 Rhodospirillum rubrum
3.2.2.24 56.6
-
 strain UR276, anaerobic preparation, prepared in the presence of 1 mM dithionite, pH 7.5 Rhodospirillum rubrum
3.2.2.24 138
-
 strain UR276, anaerobic preparation, pH 7.5 Rhodospirillum rubrum

Storage Stability

EC Number Storage Stability Organism
3.2.2.24 4°C, purified preparation exposed to air in the absence of dithionite, 22 h Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex
-
 Rhodospirillum rubrum ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?