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Literature summary extracted from
- Two DNA glycosylases in Escherichia coli which release primarily 3-methyladenine (1982), Biochemistry, 21, 1162-1169.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.21 | spermidine | stimulates activity | Escherichia coli |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.20 | 3-Methyladenine | noncompetitive inhibition | Escherichia coli | |
| 3.2.2.20 | apurinic-DNA | - |
Escherichia coli | |
| 3.2.2.20 | Double-stranded DNA | - |
Escherichia coli | |
| 3.2.2.20 | N-ethylmaleimide | 1 mM, 29% inhibition | Escherichia coli | |
| 3.2.2.20 | NaCl | - |
Escherichia coli | |
| 3.2.2.20 | spermidine | - |
Escherichia coli | |
| 3.2.2.21 | apurinic-DNA | - |
Escherichia coli | |
| 3.2.2.21 | Double-stranded DNA | - |
Escherichia coli | |
| 3.2.2.21 | additional information | resistant to product inhibition by free 3-methyladenine | Escherichia coli | |
| 3.2.2.21 | N-ethylmaleimide | 1 mM, 24% inhibition | Escherichia coli | |
| 3.2.2.21 | NaCl | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.2.20 | 0.000014 | - |
3-methyladenine residues in alkylated DNA | pH 7.8, 37°C, release of 3-methyladenine | Escherichia coli | |
| 3.2.2.21 | 0.0000092 | - |
3-methyladenine residues in alkylated DNA | pH 8.0, 37°C, release of 3-methyladenine | Escherichia coli | |
| 3.2.2.21 | 0.000011 | - |
7-methylguanine residues in alkylated DNA | pH 8.0, 37°C, release of 7-methylguanine | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.20 | Mg2+ | stimulated by 1 mM | Escherichia coli | |
| 3.2.2.21 | Mg2+ | stimulated by 1 mM | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.20 | 20000 | - |
gel filtration | Escherichia coli |
| 3.2.2.21 | 27000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.20 | alkylated DNA + H2O | Escherichia coli | - |
3-methyladenine + ? | - |
? | |
| 3.2.2.20 | alkylated DNA + H2O | Escherichia coli BW 9062 | - |
3-methyladenine + ? | - |
? | |
| 3.2.2.21 | alkylated DNA + H2O | Escherichia coli | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.20 | Escherichia coli | - |
- |
- |
| 3.2.2.20 | Escherichia coli BW 9062 | - |
- |
- |
| 3.2.2.20 | no activity in Escherichia coli | - |
mutants tag 1 and tag 2 | - |
| 3.2.2.21 | Escherichia coli | - |
- |
- |
| 3.2.2.21 | Escherichia coli BW 9062 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.20 | partially | Escherichia coli |
| 3.2.2.21 | partially | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.20 | 3-methyladenine residues in alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + ? | - |
? | |
| 3.2.2.20 | 3-methyladenine residues in alkylated DNA + H2O | - |
Escherichia coli BW 9062 | 3-methyladenine + ? | - |
? | |
| 3.2.2.20 | alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + ? | - |
? | |
| 3.2.2.20 | alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + ? | also releases a minmal amount of 3-methylguanine | ? | |
| 3.2.2.20 | alkylated DNA + H2O | - |
Escherichia coli BW 9062 | 3-methyladenine + ? | - |
? | |
| 3.2.2.20 | alkylated DNA + H2O | - |
Escherichia coli BW 9062 | 3-methyladenine + ? | also releases a minmal amount of 3-methylguanine | ? | |
| 3.2.2.20 | additional information | will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli | ? | - |
? | |
| 3.2.2.20 | additional information | will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli BW 9062 | ? | - |
? | |
| 3.2.2.21 | 3-methyladenine residues in alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + DNA | - |
? | |
| 3.2.2.21 | 7-methylguanine residues in alkylated DNA + H2O | - |
Escherichia coli | 7-methylguanine + DNA | - |
? | |
| 3.2.2.21 | alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
| 3.2.2.21 | alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + 7-methylguanine + 7-methyladenine + DNA | - |
? | |
| 3.2.2.21 | DNA treated with beta-[3H]propiolactone + H2O | - |
Escherichia coli | N1-(carboxyethyl)adenine + N7-(carboxymethyl)guanine | - |
? | |
| 3.2.2.21 | additional information | enzyme releases N1-(carboxyethyl)adenine and N7-(carboxymethyl)guanine from DNA treated with beta-propiolactone, but does not release the aflatoxin B-1 adduct at N-7 of guanine, release of 3-methyladenine from single-stranded DNA is 9% of the rate from double-stranded DNA, enzyme will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli | ? | - |
? | |
| 3.2.2.21 | additional information | enzyme releases N1-(carboxyethyl)adenine and N7-(carboxymethyl)guanine from DNA treated with beta-propiolactone, but does not release the aflatoxin B-1 adduct at N-7 of guanine, release of 3-methyladenine from single-stranded DNA is 9% of the rate from double-stranded DNA, enzyme will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli BW 9062 | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.20 | 48 | - |
50% inactivation requires 5 min | Escherichia coli |
| 3.2.2.21 | 48 | - |
50% inactivation requires 65 min | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.20 | 7 | 9 | more active in 0.07 M HEPES-KOH buffer than in 0.07 M Tris-HCl buffer | Escherichia coli |
| 3.2.2.21 | 7 | 9 | - |
Escherichia coli |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.2.20 | 1.6 | - |
3-Methyladenine | pH 7.8, 37°C | Escherichia coli | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.2.20 | Escherichia coli | isoelectric focusing | - |
6 |
| 3.2.2.21 | Escherichia coli | isoelectric focusing | 7.4 | 7.3 |
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