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Literature summary extracted from

  • Hung, T.H.; Chang, Y.M.; Sung, H.Y.; Chang, C.T.
    Purification and characterization of hydrolase with chitinase and chitosanase activity from commercial stem bromelain (2002), J. Agric. Food Chem., 50, 4666-4673.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ag+
-
 Ananas comosus
3.2.1.14 Hg2+
-
 Ananas comosus
3.2.1.14 N-bromosuccinimide
-
 Ananas comosus
3.2.1.14 p-hydroxymercuribenzoate
-
 Ananas comosus
3.2.1.132 Ag+
-
 Ananas comosus
3.2.1.132 Hg2+
-
 Ananas comosus
3.2.1.132 N-bromosuccinimide
-
 Ananas comosus
3.2.1.132 p-hydroxymercuribenzoic acid
-
 Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 additional information 0.088 mg/ml chitosan Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 15800
-
 2 * 15800, SDS-PAGE Ananas comosus
3.2.1.14 31400
-
 gel filtration Ananas comosus
3.2.1.132 15800
-
 x * 15800, SDS-PAGE Ananas comosus
3.2.1.132 31400
-
 gel filtration Ananas comosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Ananas comosus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Ananas comosus
-
-
-
3.2.1.132 Ananas comosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Ananas comosus
3.2.1.132
-
 Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 stem
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 444
-
 after Superdex 75 HR gel filtration Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Ananas comosus ?
-
 ?
3.2.1.14 chitopentaose + H2O
-
 Ananas comosus chitobiose + chitotriose + D-glucosamine
-
 ?
3.2.1.14 chitosan + H2O
-
 Ananas comosus glucosamine + chitobiose + chitooligomers with a chain length longer than 6
-
 ?
3.2.1.14 chitotetraose + H2O
-
 Ananas comosus chitobiose + chitotriose + D-glucosamine
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Ananas comosus ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Ananas comosus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
3.2.1.132 chitosan + H2O
-
 Ananas comosus ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Ananas comosus additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 dimer 2 * 15800, SDS-PAGE Ananas comosus
3.2.1.132 ? x * 15800, SDS-PAGE Ananas comosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
 with chitosan or 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside as substrate Ananas comosus
3.2.1.14 60
-
 with glycol chitin as substrate Ananas comosus
3.2.1.132 50
-
-
 Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 3
-
 with chitosan as substrate Ananas comosus
3.2.1.14 3.5 5.5 with colloidal chitin as substrate Ananas comosus
3.2.1.14 4
-
 with glycol chitin or 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside as substrate Ananas comosus
3.2.1.132 3
-
-
 Ananas comosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Ananas comosus isoelectric focusing
-
 6
3.2.1.132 Ananas comosus isoelectric focusing
-
 5.9