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Literature summary extracted from

  • Zhang, X.Y.; Dai, A.L.; Zhang, X.K.; Kuroiwa, K.; Kodaira, R.; Shimosaka, M.; Okazaki, M.
    Purification and characterization of chitosanase and exo-beta-D-glucosaminidase from a Koji mold, Aspergillus oryzae IAM2660 (2000), Biosci. Biotechnol. Biochem., 64, 1896-1902.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
Aspergillus oryzae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 40000
-
x * 40000, SDS-PAGE, enzyme I Aspergillus oryzae
3.2.1.132 135000
-
x * 135000, SDS-PAGE, enzyme II Aspergillus oryzae
3.2.1.165 135000
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Aspergillus oryzae
-
?
-
?
3.2.1.132 chitosan + H2O Aspergillus oryzae IAM2660
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Aspergillus oryzae
-
IAM2660
-
3.2.1.132 Aspergillus oryzae IAM2660
-
IAM2660
-
3.2.1.165 Aspergillus oryzae
-
-
-
3.2.1.165 Aspergillus oryzae IAM2660
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
Aspergillus oryzae
3.2.1.165
-
Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.165 culture fluid
-
Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 17.2
-
enzyme I after after Sephadex G-100 chromatography Aspergillus oryzae
3.2.1.132 38.8
-
enzyme II after after Sephadex G-100 chromatography Aspergillus oryzae
3.2.1.165 38.8
-
-
Aspergillus oryzae

Storage Stability

EC Number Storage Stability Organism
3.2.1.165 4°C, pH 5.5, sodium acetate buffer, 4 months, stable Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
Aspergillus oryzae ?
-
?
3.2.1.132 chitosan + H2O
-
Aspergillus oryzae IAM2660 ?
-
?
3.2.1.132 chitosan + H2O enzyme I prefers chitosan with 30% acetylation, but enzyme II mostly effects chitosan without acetylation Aspergillus oryzae additional information
-
?
3.2.1.132 chitosan + H2O enzyme I prefers chitosan with 30% acetylation, but enzyme II mostly effects chitosan without acetylation Aspergillus oryzae IAM2660 additional information
-
?
3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine Aspergillus oryzae D-glucosamine + ?
-
?
3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine Aspergillus oryzae IAM2660 D-glucosamine + ?
-
?
3.2.1.165 chitosan + H2O only glucosamine is produced as final product after prolonged reaction Aspergillus oryzae D-glucosamine + ?
-
?
3.2.1.165 chitosan + H2O only glucosamine is produced as final product after prolonged reaction Aspergillus oryzae IAM2660 D-glucosamine + ?
-
?
3.2.1.165 glycol chitosan + H2O 4% of the activity with chitosan Aspergillus oryzae ?
-
?
3.2.1.165 glycol chitosan + H2O 4% of the activity with chitosan Aspergillus oryzae IAM2660 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 135000, SDS-PAGE, enzyme II Aspergillus oryzae
3.2.1.132 ? x * 40000, SDS-PAGE, enzyme I Aspergillus oryzae
3.2.1.165 monomer 1 * 135000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
-
Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.165 50
-
-
Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.165 60
-
20 min, 30% loss of activity Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.165 5.5
-
-
Aspergillus oryzae