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Literature summary extracted from

  • Boucher, I.; Dupuy, A.; Vidal, P.; Neugebauer, W.A.; Brzezinski, R.
    Purification and characterization of a chitosanase from Streptomyces N174 (1992), Appl. Microbiol. Biotechnol., 38, 188-193.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 additional information 0.088 mg/ml chitosan Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Streptomyces sp.
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Streptomyces sp.
-
 N174
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 59.8
-
 after Bio-Gel A 0.5 M chromatography Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.132 chitosan + H2O the smallest of the substrates is a tetramer of glucosamine Streptomyces sp. additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 additional information no activity with chitin (i.e. 100% acetylated chitosan) Streptomyces sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 65
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 37
-
 stable Streptomyces sp.
3.2.1.132 40
-
 rapid decrease of activity above Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.5
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4 6
-
 Streptomyces sp.