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Literature summary extracted from

  • Muro, T.; Kuramoto, T.; Imoto, K.; Okada, S.
    Purification and some properties of glycyrrhizinic acid hydrolase from Aspergillus niger GRM3 (1986), Agric. Biol. Chem., 50, 687-692.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.128 150000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.128 Aspergillus niger
-
 GRM 3
-
3.2.1.128 Aspergillus niger GRM 3
-
 GRM 3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.128
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.128 additional information
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.128 glycyrrhizinic acid + H2O a pentacyclic triterpene saponin from liquorice roots, best substrate Aspergillus niger glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
-
 ?
3.2.1.128 glycyrrhizinic acid + H2O a pentacyclic triterpene saponin from liquorice roots, best substrate Aspergillus niger GRM 3 glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
-
 ?
3.2.1.128 additional information no substrates are 4-nitrophenyl beta-D-glucuronide, phenolphthalein beta-D-glucuronide Aspergillus niger ?
-
 ?
3.2.1.128 additional information no substrates are 4-nitrophenyl beta-D-glucuronide, phenolphthalein beta-D-glucuronide Aspergillus niger GRM 3 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.128 45
-
 at pH 5 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.128 30 53 about 63% of maximal activity at 30°C and about half-maximal activity at 53°C, pH 5 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.128 4.1 4.5
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.128 3 4.8 about half-maximal activity at pH 3 and 4.8 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.128 5 6.5 the enzyme is stable at 40°C Aspergillus niger