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Literature summary extracted from

  • Hasper, A.A.; Dekkers, E.; Van Mil, M.; Van de Vondervoort, P.J.I.; De Graaff, L.H.
    EglC, a new endoglucanase from Aspergillus niger with major activity towards xyloglucan (2002), Appl. Environ. Microbiol., 68, 1556-1560.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.120
-
 Aspergillus niger
3.2.1.151
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 90500
-
 calculated from amino acid sequence Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.151 additional information Aspergillus niger involved in plant cell wall polysaccharide degradation ?
-
 ?
3.2.1.151 xyloglucan + H2O Aspergillus niger
-
 xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.120 Aspergillus niger
-
-
-
3.2.1.151 Aspergillus niger Q8TFP1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.151
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.120 1
-
 pH 4.5, 40°C, substrate: chemically modified cellulose Aspergillus niger
3.2.1.120 1
-
 pH 4.5, 40°C, substrate: beta-glucan Aspergillus niger
3.2.1.120 19
-
 pH 4.5, 40°C, substrate: tamarind xyloglucan Aspergillus niger
3.2.1.151 19
-
 substrate: xyloglucan Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.120 azo-cellulose + H2O
-
 Aspergillus niger oligosaccharide + ?
-
 ?
3.2.1.120 beta-glucan + H2O
-
 Aspergillus niger oligosaccharide + ?
-
 ?
3.2.1.120 chemically modified cellulose + H2O
-
 Aspergillus niger oligosaccharide + ?
-
 ?
3.2.1.120 additional information no substrate: xylan Aspergillus niger ?
-
 ?
3.2.1.120 tamarind xyloglucan + H2O
-
 Aspergillus niger oligosaccharide + ?
-
 ?
3.2.1.151 azo-cellulose + H2O
-
 Aspergillus niger azo-cellulose oligosaccharides
-
 ?
3.2.1.151 beta-glucan + H2O
-
 Aspergillus niger beta-glucan oligosaccharides
-
 ?
3.2.1.151 additional information no substrate: xylan Aspergillus niger ?
-
 ?
3.2.1.151 additional information involved in plant cell wall polysaccharide degradation Aspergillus niger ?
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Aspergillus niger xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O best substrate Aspergillus niger xyloglucan oligosaccharides
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.120 55
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.120 30
-
 145 h, 90% residual activity Aspergillus niger
3.2.1.120 55
-
 48 h, 50% residual activity Aspergillus niger
3.2.1.151 30
-
 10% loss of activity after 145h at pH 4.5 Aspergillus niger
3.2.1.151 55
-
 80% loss of activity after 145h and 50% loss of activity after 48h at pH 4.5 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.120 4.5
-
-
 Aspergillus niger
3.2.1.151 4.5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.120 3.5 5
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 Aspergillus niger calculated from amino acid sequence
-
 4.1