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Literature summary extracted from
- Studies on enzymic production of oligosaccharides. Part VI. Maltotriose-producing amylase from Microbacterium imperiale (1991), Agric. Biol. Chem., 55, 687-692.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.116 | nutrition | enzyme produced on the industrial scale for the production of high maltotriose-containing syrup | Microbacterium imperiale |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.116 | extracellular | - |
Microbacterium imperiale | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 25500 | - |
x * 25500, SDS-PAGE | Microbacterium imperiale |
| 3.2.1.116 | 40000 | - |
gel filtration | Microbacterium imperiale |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.116 | Microbacterium imperiale | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.116 | - |
Microbacterium imperiale |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | additional information | - |
- |
Microbacterium imperiale |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.116 | 3 glycogen + H2O | - |
Microbacterium imperiale | 4 maltotriose | main product | ? |  |
| 3.2.1.116 | amylopectin + H2O | - |
Microbacterium imperiale | maltotriose | main product | ? | |
| 3.2.1.116 | amylose + H2O | - |
Microbacterium imperiale | maltotriose | main product | ? | |
| 3.2.1.116 | beta-cyclodextrin + H2O | - |
Microbacterium imperiale | maltotriose | main product | ? | |
| 3.2.1.116 | maltohexaose + H2O | - |
Microbacterium imperiale | maltotriose + ? | alpha-configuration | ? | |
| 3.2.1.116 | maltooligosaccharide + H2O | hydrolysis of maltohexaose, maltopentaose, maltotetraose and maltotriose | Microbacterium imperiale | maltotriose | alpha-configuration | ? | |
| 3.2.1.116 | maltopentaose + H2O | - |
Microbacterium imperiale | maltotriose + D-maltose | alpha-configuration | ? | |
| 3.2.1.116 | maltotetraose + H2O | - |
Microbacterium imperiale | maltotriose + D-glucose | alpha-configuration, additional product: D-maltose | ? | |
| 3.2.1.116 | maltotriose + H2O | slow hydrolysis | Microbacterium imperiale | maltose + D-glucose | alpha-configuration | ? | |
| 3.2.1.116 | additional information | not: alpha-cyclodextrin, pullulan | Microbacterium imperiale | ? | - |
? | |
| 3.2.1.116 | additional information | no hydrolysis of alpha-1,6-glucosidic linkages | Microbacterium imperiale | ? | - |
? | |
| 3.2.1.116 | starch + H2O | soluble | Microbacterium imperiale | maltotriose + ? | initial main product, proceeded reaction results in 55-60% maltotriose, 10-20% maltose, 1-3% glucose, 20-40% higher oligosaccharides | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.116 | ? | x * 25500, SDS-PAGE | Microbacterium imperiale |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 40 | - |
assay at | Microbacterium imperiale |
| 3.2.1.116 | 50 | - |
30 min | Microbacterium imperiale |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 25 | 55 | about 50% of activity maximum at 25°C and 55°C | Microbacterium imperiale |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | additional information | - |
Ca2+ protects against heat inactivation | Microbacterium imperiale |
| 3.2.1.116 | 40 | - |
unstable above | Microbacterium imperiale |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 6.5 | 7 | - |
Microbacterium imperiale |
| 3.2.1.116 | 7 | - |
assay at | Microbacterium imperiale |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 5.8 | 8.5 | pH 5.8: about 90% of activity maximum, pH 8.5: about 30% of activity maximum | Microbacterium imperiale |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 5 | 9 | stable in the pH range | Microbacterium imperiale |
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