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Literature summary extracted from

  • Canellakis, Z.N.; Bondy, P.K.; May, Jr., J.A.; Myers-Robfogel, M.K.; Sartorelli, A.C.
    Indentification of a glycosidase activity with apparent specificity for 2-deoxy-D-glucose in glycosidic linkage (1984), Eur. J. Biochem., 143, 159-163.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.112 medicine potential target to increase effectiveness of 2-deoxy-D-glucose in chemotherapy and viral infections Mus musculus

General Stability

EC Number General Stability Organism
3.2.1.112 all stages of purification can be stored at -20°C for 18 months with no loss of activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.112 D-mannono-1,5-lactone
-
 Mus musculus
3.2.1.112 D-mannono-1,5-lactone
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.112 2
-
 p-nitrophenyl-2-deoxy-alpha-D-glucoside pH 7, 37°C Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.112 H2O + a 2-deoxy-alpha-D-glucoside Mus musculus physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules 2-deoxy-D-glucose + an alcohol
-
 r
3.2.1.112 H2O + a 2-deoxy-alpha-D-glucoside Rattus norvegicus physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules 2-deoxy-D-glucose + an alcohol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.112 Mus musculus
-
 P288 leukemia cells
-
3.2.1.112 Rattus norvegicus
-
 HTC cells and liver acetone powder
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.112 partial Mus musculus
3.2.1.112 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.112 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.112 0.035
-
 partially purified enzyme, pH 7, 37°C Mus musculus

Storage Stability

EC Number Storage Stability Organism
3.2.1.112 -20°C, 18 months, no loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.112 H2O + a 2-deoxy-alpha-D-glucoside
-
 Mus musculus 2-deoxy-D-glucose + an alcohol
-
 r
3.2.1.112 H2O + a 2-deoxy-alpha-D-glucoside
-
 Rattus norvegicus 2-deoxy-D-glucose + an alcohol
-
 r
3.2.1.112 H2O + a 2-deoxy-alpha-D-glucoside physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules Mus musculus 2-deoxy-D-glucose + an alcohol
-
 r
3.2.1.112 H2O + a 2-deoxy-alpha-D-glucoside physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules Rattus norvegicus 2-deoxy-D-glucose + an alcohol
-
 r
3.2.1.112 H2O + p-nitrophenyl-2-deoxy-alpha-D-glucoside
-
 Mus musculus 2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.112 H2O + p-nitrophenyl-2-deoxy-alpha-D-glucoside
-
 Rattus norvegicus 2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.112 H2O + p-nitrophenyl-alpha-D-glucoside poor substrate Rattus norvegicus alpha-D-glucose + p-nitrophenol
-
 ?
3.2.1.112 H2O + p-nitrophenyl-alpha-D-mannoside poor substrate Mus musculus alpha-D-mannose + p-nitrophenol
-
 ?
3.2.1.112 H2O + p-nitrophenyl-alpha-D-mannoside poor substrate Rattus norvegicus alpha-D-mannose + p-nitrophenol
-
 ?
3.2.1.112 H2O + p-nitrophenyl-beta-D-glucoside poor substrate Rattus norvegicus beta-D-glucose + p-nitrophenol
-
 ?
3.2.1.112 H2O + p-nitrophenyl-beta-N-acetyl-D-glucosaminoside
-
 Mus musculus beta-N-acetyl-D-glucosamine + p-nitrophenol
-
 ?
3.2.1.112 H2O + p-nitrophenyl-beta-N-acetyl-D-glucosaminoside
-
 Rattus norvegicus beta-N-acetyl-D-glucosamine + p-nitrophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.112 50
-
 stable for at least 5 min Mus musculus
3.2.1.112 60
-
 98% loss of activity within 5 min Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.112 6.5 8.5 much lower activity with Tris than with HCl/KCl buffer Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.112 4 10 much lower activity with Tris than with HCl/KCl buffer Mus musculus