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Literature summary extracted from

  • Freeman, C.; Hopwood, J.J.
    Glucuronate-2-sulphatase activity in cultured human skin fibroblast homogenates (1991), Biochem. J., 279, 399-405.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.6.18 bovine serum albumin activation Homo sapiens
3.1.6.18 bovine serum albumin over pH-range 2.7-5.7, maximal activation at 1 mg/ml and pH 3.3, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.18 Cu2+ above pH 4.2, activation over pH-range 3-4.2, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate Homo sapiens
3.1.6.18 EDTA glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate Homo sapiens
3.1.6.18 NaCl at pH 3, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate; stimulates between pH 4.2-4.8 Homo sapiens
3.1.6.18 NaH2PO4 glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate Homo sapiens
3.1.6.18 SO42- glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate; product inhibition Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.6.18 Cu2+ activation Homo sapiens
3.1.6.18 Cu2+ pH-dependent: over pH-range 3-4.2, inhibits at higher pH, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate Homo sapiens
3.1.6.18 NaCl stimulation, over pH-range 4.2-4.8, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate, inhibits at pH 3 Homo sapiens
3.1.6.18 Zn2+ activation Homo sapiens
3.1.6.18 Zn2+ over pH-range 3-4.8 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.18 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.18 skin fibroblast cell culture Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.18 D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O i.e. O-beta-glucuronic acid 2-sulfate-1,4-O-(2,5)-anhydromannitol 6-sulfate Homo sapiens D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42-
-
 ?
3.1.6.18 D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O i.e. GSMS Homo sapiens D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42-
-
 ?
3.1.6.18 D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O i.e. O-beta-glucuronic acid 2-sulfate-1,4-O-(2,5)-anhydrotalitol 6-sulfate Homo sapiens D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42-
-
 ?
3.1.6.18 D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O i.e. GSTS Homo sapiens D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42-
-
 ?
3.1.6.18 additional information no hydrolysis of iduronate 2-sulfate residues Homo sapiens ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.18 additional information
-
 the pH/activity profile depends on buffer composition and ionic strength, particularly at lower pH-values Homo sapiens
3.1.6.18 2.7
-
 20 mM sodium formate buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate as substrate Homo sapiens
3.1.6.18 3
-
 glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate Homo sapiens
3.1.6.18 3
-
 4-20 mM sodium formate or 20-60 mM sodium acetate buffer Homo sapiens
3.1.6.18 3.3
-
 60 mM sodium formate or 60 mM glycine/HCl buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate Homo sapiens