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Literature summary extracted from
- Purification of an inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase activity from rat liver and the evaluation of its substrate specificity (1991), J. Biol. Chem., 266, 16499-16506.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.62 | hemoglobin | from turkey, activates by removing the inhibitors inositol 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.62 | Inositol 1,3,4,5,6-pentakisphosphate | competitive; strong, kinetics; turkey hemoglobin reverses | Rattus norvegicus |  |
| 3.1.3.62 | inositol 1,3,4-trisphosphate | - |
Rattus norvegicus | |
| 3.1.3.62 | Inositol 1,4,5-trisphosphate | product inhibition kinetics | Rattus norvegicus | |
| 3.1.3.62 | inositol 3,4,5,6-tetrakisphosphate | - |
Rattus norvegicus | |
| 3.1.3.62 | Inositol hexakisphosphate | competitive, turkey hemoglobin reverses; more effective than inositol pentakisphosphate; strong, kinetics | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.62 | 0.0000003 | - |
Inositol hexakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
| 3.1.3.62 | 0.00004 | - |
Inositol 1,3,4,5,6-pentakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
| 3.1.3.62 | 0.00013 | - |
D-myo-Inositol 1,3,4,5-tetrakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.62 | particle-bound | predominantly in particulate fraction | Rattus norvegicus | - |
- |
| 3.1.3.62 | soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.62 | Mg2+ | 2.5fold activation at 5-10 mM, no activation at 1 mM and physiologically relevant concentrations | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.62 | 66000 | - |
particulate enzyme, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.62 | inositol 1,3,4,5,6-pentakisphosphate + H2O | Rattus norvegicus | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | inositol 1,4,5,6-tetrakisphosphate + phosphate | - |
? | |
| 3.1.3.62 | inositol hexakisphosphate + H2O | Rattus norvegicus | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | inositol pentakisphosphate + phosphate | - |
? | |
| 3.1.3.62 | additional information | Rattus norvegicus | enzyme is relatively unimportant to inositol 1,3,4,5-tetrakisphosphate metabolism in vivo | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.62 | Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.62 | particulate enzyme: 38374fold, soluble enzyme: partial | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.62 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.62 | 4.66 | - |
pH 7.4, 37°C, membrane-bound enzyme | Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.62 | -80°C, at least 3 months | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.62 | D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Rattus norvegicus | D-myo-inositol 1,4,5-trisphosphate + phosphate | - |
? | |
| 3.1.3.62 | inositol 1,3,4,5,6-pentakisphosphate + H2O | - |
Rattus norvegicus | inositol 1,4,5,6-tetrakisphosphate + phosphate | - |
? | |
| 3.1.3.62 | inositol 1,3,4,5,6-pentakisphosphate + H2O | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | Rattus norvegicus | inositol 1,4,5,6-tetrakisphosphate + phosphate | - |
? | |
| 3.1.3.62 | inositol 1,3,4,6-tetrakisphosphate + H2O | very poor substrate | Rattus norvegicus | ? | - |
? | |
| 3.1.3.62 | inositol 3,4,5,6-tetrakisphosphate + H2O | about 10% of activity with inositol 1,3,4,5-tetrakisphosphate | Rattus norvegicus | ? | - |
? | |
| 3.1.3.62 | inositol hexakisphosphate + H2O | - |
Rattus norvegicus | inositol pentakisphosphate + phosphate | at least 4 isomers: D- and/or L-inositol 1,2,4,5,6-pentakisphosphate, D-inositol 1,3,4,5,6-pentakisphosphate, D-inositol 1,2,3,4,6-pentakisphosphate and D- and/or L-inositol 1,2,3,4,5-pentakisphosphate | ? | |
| 3.1.3.62 | inositol hexakisphosphate + H2O | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | Rattus norvegicus | inositol pentakisphosphate + phosphate | - |
? | |
| 3.1.3.62 | additional information | not: inositol 1,3,4-trisphosphate, inositol 1,3-bisphosphate, inositol 3,4-bisphosphate, 4-nitrophenyl phosphate | Rattus norvegicus | ? | - |
? | |
| 3.1.3.62 | additional information | enzyme is relatively unimportant to inositol 1,3,4,5-tetrakisphosphate metabolism in vivo | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.62 | monomer | 1 * 66000, particulate enzyme, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.62 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.62 | 5 | 9 | plateau within the range, inositol 1,3,4,5,6-pentakisphosphate or inositol hexakisphosphate as substrate, in absence of Mg2+ | Rattus norvegicus |
| 3.1.3.62 | 6.1 | - |
inositol 1,3,4,5-tetrakisphosphate as substrate, in absence of Mg2+ | Rattus norvegicus |
| 3.1.3.62 | 7.4 | - |
inositol hexakisphosphate as substrate, plus 1 mM Mg2+ | Rattus norvegicus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.62 | 0.0000005 | - |
Inositol hexakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
| 3.1.3.62 | 0.000025 | - |
Inositol 1,3,4,5,6-pentakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
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