BRENDA - Enzyme Database

1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase activity in Tetrahymena pyriformis

Tellis, C.; Lekka, M.E.; J. Eukaryot. Microbiol. 47, 122-128 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.59
Tween 20
20% increase of activity
Tetrahymena pyriformis
3.1.3.59
Tween 80
20% increase of activity
Tetrahymena pyriformis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.59
1-chloro-2,3-dinitrobenzene
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
3.1.3.59
acyl-glycerolphosphate
38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
3.1.3.59
alkyl-glycerolphosphate
58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
3.1.3.59
Ca2+
-
Tetrahymena pyriformis
3.1.3.59
dithiothreitol
-
Tetrahymena pyriformis
3.1.3.59
Mg2+
-
Tetrahymena pyriformis
3.1.3.59
additional information
no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
Tetrahymena pyriformis
3.1.3.59
N-ethylmaleimide
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
3.1.3.59
Nonidet
100% inhibition
Tetrahymena pyriformis
3.1.3.59
SDS
100% inhibition
Tetrahymena pyriformis
3.1.3.59
sodium deoxycholate
100% inhibition
Tetrahymena pyriformis
3.1.3.59
Triton-X100
0.2% causes 100% inhibition
Tetrahymena pyriformis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.59
0.032
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
pH 7.0, 37°C, mitochondrial and microsomal fractions
Tetrahymena pyriformis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.59
cytosol
15% of the total activity
Tetrahymena pyriformis
5829
-
3.1.3.59
membrane
-
Tetrahymena pyriformis
16020
-
3.1.3.59
microsome
20% of the total activity
Tetrahymena pyriformis
-
-
3.1.3.59
mitochondrion
40% of the total activity
Tetrahymena pyriformis
5739
-
3.1.3.59
pellicle
20% of the total activity
Tetrahymena pyriformis
20039
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.59
additional information
Tetrahymena pyriformis
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
?
-
-
-
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.3.59
additional information
EDTA and EGTA no affect on enzyme activity
Tetrahymena pyriformis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.59
Tetrahymena pyriformis
-
protozoan
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.59
additional information
-
-
Tetrahymena pyriformis
3.1.3.59
0.0031
-
cytosol
Tetrahymena pyriformis
3.1.3.59
0.0159
-
pellicle
Tetrahymena pyriformis
3.1.3.59
0.0164
-
mitochondrion and microsome
Tetrahymena pyriformis
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.59
-80°C, stable for 3 months
Tetrahymena pyriformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.59
1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
-
646420
Tetrahymena pyriformis
1-acyl-2-acetyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.59
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
best substrate
646420
Tetrahymena pyriformis
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
646420
Tetrahymena pyriformis
?
3.1.3.59
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
-
646420
Tetrahymena pyriformis
?
-
-
-
?
3.1.3.59
alkyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
alkylglycerol + phosphate
-
-
-
?
3.1.3.59
diacyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
dialkylglycerol + phosphate
poor substrate
646420
Tetrahymena pyriformis
?
3.1.3.59
additional information
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
646420
Tetrahymena pyriformis
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.59
20
45
stable
Tetrahymena pyriformis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.59
7
-
microsome and mitochondrion
Tetrahymena pyriformis
3.1.3.59
9
-
microsome
Tetrahymena pyriformis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.59
Tween 20
20% increase of activity
Tetrahymena pyriformis
3.1.3.59
Tween 80
20% increase of activity
Tetrahymena pyriformis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.59
1-chloro-2,3-dinitrobenzene
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
3.1.3.59
acyl-glycerolphosphate
38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
3.1.3.59
alkyl-glycerolphosphate
58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
3.1.3.59
Ca2+
-
Tetrahymena pyriformis
3.1.3.59
dithiothreitol
-
Tetrahymena pyriformis
3.1.3.59
Mg2+
-
Tetrahymena pyriformis
3.1.3.59
additional information
no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
Tetrahymena pyriformis
3.1.3.59
N-ethylmaleimide
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
3.1.3.59
Nonidet
100% inhibition
Tetrahymena pyriformis
3.1.3.59
SDS
100% inhibition
Tetrahymena pyriformis
3.1.3.59
sodium deoxycholate
100% inhibition
Tetrahymena pyriformis
3.1.3.59
Triton-X100
0.2% causes 100% inhibition
Tetrahymena pyriformis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.59
0.032
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
pH 7.0, 37°C, mitochondrial and microsomal fractions
Tetrahymena pyriformis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.59
cytosol
15% of the total activity
Tetrahymena pyriformis
5829
-
3.1.3.59
membrane
-
Tetrahymena pyriformis
16020
-
3.1.3.59
microsome
20% of the total activity
Tetrahymena pyriformis
-
-
3.1.3.59
mitochondrion
40% of the total activity
Tetrahymena pyriformis
5739
-
3.1.3.59
pellicle
20% of the total activity
Tetrahymena pyriformis
20039
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.59
additional information
Tetrahymena pyriformis
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
?
-
-
-
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.3.59
additional information
EDTA and EGTA no affect on enzyme activity
Tetrahymena pyriformis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.59
additional information
-
-
Tetrahymena pyriformis
3.1.3.59
0.0031
-
cytosol
Tetrahymena pyriformis
3.1.3.59
0.0159
-
pellicle
Tetrahymena pyriformis
3.1.3.59
0.0164
-
mitochondrion and microsome
Tetrahymena pyriformis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.59
-80°C, stable for 3 months
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.59
1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
-
646420
Tetrahymena pyriformis
1-acyl-2-acetyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.59
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
best substrate
646420
Tetrahymena pyriformis
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
646420
Tetrahymena pyriformis
?
3.1.3.59
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
-
646420
Tetrahymena pyriformis
?
-
-
-
?
3.1.3.59
alkyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
alkylglycerol + phosphate
-
-
-
?
3.1.3.59
diacyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
dialkylglycerol + phosphate
poor substrate
646420
Tetrahymena pyriformis
?
3.1.3.59
additional information
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
646420
Tetrahymena pyriformis
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.59
20
45
stable
Tetrahymena pyriformis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.59
7
-
microsome and mitochondrion
Tetrahymena pyriformis
3.1.3.59
9
-
microsome
Tetrahymena pyriformis