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Literature summary extracted from

  • Lee, T.C.; Malone, B.; Snyder, F.
    Formation of 1-alkyl-2-acetyl-sn-glycerols via the de novo biosynthetic pathway for platelet-activating factor. Characterization of 1-alkyl-2-acetyl-sn-glycero-3-phosphate phosphohydrolase in rat spleens (1988), J. Biol. Chem., 263, 1755-1760.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.59 Ca2+ above 0.5 mM Rattus norvegicus
3.1.3.59 Mg2+ above 0.5 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.59 0.0318
-
 1-Alkyl-2-acetyl-sn-glycero-3-phosphate pH 6.6-7.0, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.59 microsome 53% of the total activity Rattus norvegicus
-
-
3.1.3.59 mitochondrion 28% of the total activity Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.59 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.59 brain
-
 Rattus norvegicus
-
3.1.3.59 kidney medulla and cortex Rattus norvegicus
-
3.1.3.59 lung
-
 Rattus norvegicus
-
3.1.3.59 spleen
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.59 1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O no substrate selectivity with regard to variations in alkyl chain length, C16:0 versus C18:0, at the sn-1 position or short chain acyl groups, C2:0 to C6:0 with the exception of C3:0, at the sn-2 position of the glycerol moiety Rattus norvegicus 1-alkyl-2-acetyl-sn-glycerol + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.59 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.59 80
-
 activity destroyed Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.59 6.6 7 in homogenates and microsomal fractions of rat spleens, when ethanol is not included in the assay mixture Rattus norvegicus
3.1.3.59 7.4
-
 microsomal fraction in 5% ethanol Rattus norvegicus