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Literature summary extracted from

  • Thompson, L.D.; Daniels, C.J.
    A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties (1988), J. Biol. Chem., 263, 17951-17959.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.16 spermidine required Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.16 Ca2+ Mg2+ or Ca2+ required Haloferax volcanii
4.6.1.16 Mg2+ required Haloferax volcanii
4.6.1.16 Mg2+ Mg2+ or Ca2+ required Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.16 tRNA precursor Haloferax volcanii
-
 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.16 Haloferax volcanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.16 partial Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.16 tRNA precursor
-
 Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor precise and accurate excision of the intron from halobacterial tRNATrp precursor Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor specific for halobacterial tRNATrp substrates, does not remove the intron from a yeast pre-tRNAPhe substrate Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor does not require intact mature tRNA structure in the substrate Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?